Intercellular contact-dependent survival of human A549, NCI-H596 and NCI-H520 non-small cell lung carcinoma cell lines
dc.contributor.author | Castro, Mauro Antônio Alves | pt_BR |
dc.contributor.author | Schwartsmann, Gilberto | pt_BR |
dc.contributor.author | Moreira, Jose Claudio Fonseca | pt_BR |
dc.date.accessioned | 2010-04-24T04:15:28Z | pt_BR |
dc.date.issued | 2001 | pt_BR |
dc.identifier.issn | 0100-879X | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/21138 | pt_BR |
dc.description.abstract | In the present study, we examined the relationship between cell phenotype and cell survival of three human non-small cell lung carcinoma cell lines (A549, NCI-H596 and NCI-H520). Cells in exponential growth at various densities were incubated for 24 h at 37ºC in a 5% CO2 humidified atmosphere and then exposed to UV radiation for 1 min (256 nm, 40 W, source-to-target distance 100 cm). After two days the surviving cells were quantified by sulforhodamine ß staining and DNA fragmentation assay. The differences in UV sensitivity at 60 x 103 cells/cm2 among the cell lines were not related to the proliferative state of the cells but to the extent of intercellular contact. In contrast to A549 and NCI-H596, irradiated NCI-H520 cells presented lower DNA fragmentation and an aggregated cell culture phenotype even prior to confluence, suggesting that a contact-effect mechanism provides further protection against UV radiation. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 34, no. 8 (Aug. 2001), p. 1007-1013 | pt_BR |
dc.rights | Open Access | en |
dc.subject | Non-small cell lung carcinoma | en |
dc.subject | Neoplasias pulmonares | pt_BR |
dc.subject | Cell density | en |
dc.subject | Carcinoma pulmonar de células não pequenas | pt_BR |
dc.subject | Intercellular contact | en |
dc.subject | Contagem de células | pt_BR |
dc.subject | Ciclo celular | pt_BR |
dc.subject | Cell phenotype | en |
dc.subject | Fenótipo | pt_BR |
dc.subject | UV radiation | en |
dc.subject | Tolerância à radiação | pt_BR |
dc.title | Intercellular contact-dependent survival of human A549, NCI-H596 and NCI-H520 non-small cell lung carcinoma cell lines | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 000327080 | pt_BR |
dc.type.origin | Nacional | pt_BR |
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