Mostrar el registro sencillo del ítem

dc.contributor.authorWeber, Raquelpt_BR
dc.contributor.authorBertoni, Ana Paula Santinpt_BR
dc.contributor.authorBessestil, Laura Walterpt_BR
dc.contributor.authorAssis Brasil, Beatriz Maria de Azevedopt_BR
dc.contributor.authorBrum, Ilma Simonipt_BR
dc.contributor.authorFurlanetto, Tania Weberpt_BR
dc.date.accessioned2018-03-15T02:32:12Zpt_BR
dc.date.issued2014pt_BR
dc.identifier.issn2314-6141pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/173420pt_BR
dc.description.abstractAlternaria sp. MG1, an endophytic fungus isolated from Vitis vinifera, can independently produce resveratrol, indicating that this species contains the key genes for resveratrol biosynthesis. Identification of these key genes is essential to understand the resveratrol biosynthesis pathway in this strain, which is currently unknown in microorganisms. qRT-PCR is an efficient and widely used method to identify the key genes related to unknown pathways at the level of gene expression. Verification of stable reference genes in this strain is essential for qRT-PCR data normalization, although results have been reported for other Alternaria sp. strains. In this study, nine candidate reference genes including TUBA, EF1, EF2, UBC, UFD, RPS5, RPS24, ACTB and 18S were evaluated for expression stability in a diverse set of six samples representing different growth periods. We compared cell culture conditions and an optimized condition for resveratrol production. The comparison of the results was performed using four statistical softwares. A combination of TUBA and EF1 was found to be suitable for normalization of Alternaria sp. MG1 in different developmental stages, and 18S was found to be the least stable. The reference genes verified in this study will facilitate further research to explore gene expression and molecular mechanisms as well as the improvement of secondary metabolite yields in Alternaria sp. MG1. To our knowledge, this is the first validation of reference genes in Alternaria with the capability to produce resveratrol. Additionally, these results provide useful guidelines for the selection of reference genes in other Alternaria species.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBiomed Research International. New York. Volume 2014 (2014), ID 198582, 5 p.pt_BR
dc.rightsOpen Accessen
dc.subjectqRT-PCRen
dc.subjectExpressão gênicapt_BR
dc.subjectReference genesen
dc.subjectGenéticapt_BR
dc.subjectBóciopt_BR
dc.subjectα-Tubulinen
dc.subjectElongation factor 1en
dc.subjectHumanospt_BR
dc.subjectPadrões de referênciapt_BR
dc.subjectAlternaria spen
dc.subjectReprodutibilidade dos testespt_BR
dc.subjectReação em cadeia da polimerase via transcriptase reversapt_BR
dc.subjectGlândula tireóidept_BR
dc.subjectMetabolismopt_BR
dc.subjectMétodospt_BR
dc.titleValidation of reference genes for normalization gene expression in reverse transcription quantitative PCR in human normal thyroid and goiter tissuept_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001055267pt_BR
dc.type.originEstrangeiropt_BR


Ficheros en el ítem

Thumbnail
   

Este ítem está licenciado en la Creative Commons License

Mostrar el registro sencillo del ítem