Analysis of flavonoids in Rubus erythrocladus and Morus migra leaves extracts by liquid chromatography and capillary electrophoresis

Abstract

This study uses high performance liquid chromatography and capillary electrophoresis as analytical toolsto evaluate flavonoids in hydrolyzed leaves extracts of Rubus erythrocladus Mart., Rosaceae, and Morusnigra L., Moraceae. For phytochemical analysis, the extracts were prepared by acid hydrolysis and ultra-sonic bath and analyzed by high performance liquid chromatography using an ultraviolet detector andby capillary electrophoresis equipped with a diode-array detector. Quercetin and kaempferol were iden-tified in these extracts. The analytical methods developed were validated and applied. Quercetin andkaempferol were quantified in R. erythrocladus, with 848.43 ± 66.68 g g−1and 304.35 ± 17.29 g g−1,respectively, by HPLC-UV and quercetin, 836.37 ± 149.43 g g−1, by CE-DAD. In M. nigra the quan-tifications of quercetin and kaempferol were 2323.90 ± 145.35 g g−1and 1446.36 ± 59.00 g g−1,respectively, by HPLC-UV and, 2552.82 ± 275.30 g g−1and 1188.67 ± 99.21 g g−1, respectively, by CE-DAD. The extracts were also analyzed by ultra-performance liquid chromatography coupled with adiode-array detector and mass spectrometer (MS), UPLC-DAD/MS.