Analysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalis
dc.contributor.author | Frasson, Amanda Piccoli | pt_BR |
dc.contributor.author | Charão, Mariele Feiffer | pt_BR |
dc.contributor.author | Rosemberg, Denis Broock | pt_BR |
dc.contributor.author | Souza, Ana Paula de | pt_BR |
dc.contributor.author | Garcia, Solange Cristina | pt_BR |
dc.contributor.author | Bonorino, Cristina Beatriz Cazabuena | pt_BR |
dc.contributor.author | Bogo, Mauricio Reis | pt_BR |
dc.contributor.author | De Carli, Geraldo Attilio | pt_BR |
dc.contributor.author | Tasca, Tiana | pt_BR |
dc.date.accessioned | 2013-06-07T01:45:24Z | pt_BR |
dc.date.issued | 2012 | pt_BR |
dc.identifier.issn | 0074-0276 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/72280 | pt_BR |
dc.description.abstract | Trichomonas vaginalis is a parasite of the human urogenital tract that causes trichomonosis, the most prevalent non-viral sexually transmitted disease. Ectonucleoside triphosphate diphosphohydrolase (NTPDase) family mem- bers, which hydrolyse extracellular ATP and ADP and ecto-5′-nucleotidase, which hydrolyses AMP, have been char- acterised in T. vaginalis. For trichomonad culture, the growth medium is supplemented with 10% serum, which is an important source of nutrients, such as adenosine. Here, we investigated the ATP metabolism of T. vaginalis tropho- zoites from long-term cultures and clinical isolates under limited bovine serum conditions (1% serum). The specific enzymatic activities were expressed as nmol inorganic phosphate (Pi) released/min/mg protein, the gene expression patterns were determined by reverse transcriptase-polymerase chain reaction, the extracellular adenine nucleotide hydrolysis was analysed by high performance liquid chromatography and the cell cycle analysis was assessed by f low cytometry. Serum limitation led to the profound activation of NTPDase and ecto-5’-nucleotidase activities. Furthermore, the levels of NTPDase A and B transcripts increased and extracellular ATP metabolism was activated, which led to enhanced ATP hydrolysis and the formation of ADP and AMP. Moreover, the cell cycle was arrested at the G0/G1 stage, which suggested adenosine uptake. Our data suggest that under conditions of serum limitation, NTPDase and ecto-5’-nucleotidase play a role in providing the adenosine required for T. vaginalis growth and that this process contributes to the establishment of parasitism. | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Memórias do Instituto Oswaldo Cruz : an international journal of biological and biomedical research. Vol. 107, n. 2 (mar. 2012), p. 170-177 | pt_BR |
dc.rights | Open Access | en |
dc.subject | Trichomonas vaginalis | en |
dc.subject | Trichomonas vaginalis | pt_BR |
dc.subject | Serum limitation | en |
dc.subject | Ecto-5'-nucleotidase | pt_BR |
dc.subject | Adenosina | pt_BR |
dc.subject | Ectonucleoside triphosphate diphosphohydrolase | en |
dc.subject | Ecto-5’ | en |
dc.subject | Nucleotidase | en |
dc.subject | Adenosine | en |
dc.title | Analysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalis | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 000873516 | pt_BR |
dc.type.origin | Nacional | pt_BR |
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