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dc.contributor.authorWolff, Fernando Herzpt_BR
dc.contributor.authorFuchs, Sandra Cristina Pereira Costapt_BR
dc.contributor.authorBrandao, Ajacio Bandeira de Mellopt_BR
dc.date.accessioned2012-03-24T01:24:35Zpt_BR
dc.date.issued2011pt_BR
dc.identifier.issn1413-8670pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/37933pt_BR
dc.description.abstractBackground: Occult hepatitis B virus (HBV) infection is characterized by the detection of HBV DNA in serum and/or in liver in the absence of detectable hepatitis B surface antigen (HBsAg). The reported prevalence of occult hepatitis B varies markedly among populations and according to the sensitivity of the HBV DNA assay. The aim of the present study was to describe the prevalence of occult hepatitis B among HCV-infected and non-infected blood donors in Porto Alegre, Southern Brazil, using a highly sensitive real time polymerase chain reaction (PCR) method. Methodology: Between 1995 and 1997 a sample of 178 blood donors with two positive anti-HCV ELISA tests were consecutively selected as cases, and 356 anti-HCV negative donors were selected as controls. Blood donors were randomly selected from eight blood centers in Porto Alegre, Southern Brazil, representative of the whole blood donor population. Blood samples were kept at 70°C and defrosted for the first time for the analysis of this report. Tests previously performed in the laboratory using the same real time PCR for HBV DNA had sensitivity for detecting as low as 9 copies/mL. Among 158 blood samples from HBsAg-negative blood donors, five were anti-HBc positive, 53 tested positive for anti-HCV and 105 had anti-HCV negative. The samples analysis was performed in duplicate and all blood samples tested negative for HBV DNA. Conclusion: The result reflects a very low prevalence of occult hepatitis B in our setting.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofThe Brazilian journal of infectious diseases. Vol. 15, no. 2 (2011), p. 159-162pt_BR
dc.rightsOpen Accessen
dc.subjectHepatitis Ben
dc.subjectHepatite Bpt_BR
dc.subjectVirus da hepatite Bpt_BR
dc.subjectHepatitis B virusen
dc.subjectDoadores de sanguept_BR
dc.subjectBlood donorsen
dc.subjectSeroepidemiologic studiesen
dc.subjectEstudos soroepidemiológicospt_BR
dc.titleAbsence of occult hepatitis B among blood donors in southern Brazilpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000783781pt_BR
dc.type.originEstrangeiropt_BR


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