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Comparison of two laboratory-developed PCR methods for the diagnosis of pulmonary tuberculosis in Brazilian patients with and without HIV infection
dc.contributor.author | Scherer, Luciene Cardoso | pt_BR |
dc.contributor.author | Sperhacke, Rosa Dea | pt_BR |
dc.contributor.author | Jarczewski, Carla Adriane | pt_BR |
dc.contributor.author | Cafrune, Patricia Izquierdo | pt_BR |
dc.contributor.author | Michelon, Candice Tosi | pt_BR |
dc.contributor.author | Ruppenthal, Rubia Denise | pt_BR |
dc.contributor.author | Ribeiro, Marta Osório | pt_BR |
dc.contributor.author | Ruffino-Netto, Antonio | pt_BR |
dc.contributor.author | Rossetti, Maria Lucia Rosa | pt_BR |
dc.contributor.author | Kritski, Afrânio Lineu | pt_BR |
dc.date.accessioned | 2011-08-09T06:01:06Z | pt_BR |
dc.date.issued | 2011 | pt_BR |
dc.identifier.issn | 1471-2466 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/30950 | pt_BR |
dc.description.abstract | Background: Direct smear examination with Ziehl-Neelsen (ZN) staining for the diagnosis of pulmonary tuberculosis (PTB) is cheap and easy to use, but its low sensitivity is a major drawback, particularly in HIV seropositive patients. As such, new tools for laboratory diagnosis are urgently needed to improve the case detection rate, especially in regions with a high prevalence of TB and HIV. Objective: To evaluate the performance of two in house PCR (Polymerase Chain Reaction): PCR dot-blot methodology (PCR dot-blot) and PCR agarose gel electrophoresis (PCR-AG) for the diagnosis of Pulmonary Tuberculosis (PTB) in HIV seropositive and HIV seronegative patients. Methods: A prospective study was conducted (from May 2003 to May 2004) in a TB/HIV reference hospital. Sputum specimens from 277 PTB suspects were tested by Acid Fast Bacilli (AFB) smear, Culture and in house PCR assays (PCR dot-blot and PCR-AG) and their performances evaluated. Positive cultures combined with the definition of clinical pulmonary TB were employed as the gold standard. Results: The overall prevalence of PTB was 46% (128/277); in HIV+, prevalence was 54.0% (40/74). The sensitivity and specificity of PCR dot-blot were 74% (CI 95%; 66.1%-81.2%) and 85% (CI 95%; 78.8%-90.3%); and of PCR-AG were 43% (CI 95%; 34.5%-51.6%) and 76% (CI 95%; 69.2%-82.8%), respectively. For HIV seropositive and HIV seronegative samples, sensitivities of PCR dot-blot (72% vs 75%; p = 0.46) and PCR-AG (42% vs 43%; p = 0.54) were similar. Among HIV seronegative patients and PTB suspects, ROC analysis presented the following values for the AFB smear (0.837), Culture (0.926), PCR dot-blot (0.801) and PCR-AG (0.599). In HIV seropositive patients, these area values were (0.713), (0.900), (0.789) and (0.595), respectively. Conclusion: Results of this study demonstrate that the in house PCR dot blot may be an improvement for ruling out PTB diagnosis in PTB suspects assisted at hospitals with a high prevalence of TB/HIV. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | BMC Pulmonary medicine. London. Vol, 11, no. 15 (29 mar. 2011), 10 p. | pt_BR |
dc.rights | Open Access | en |
dc.subject | Tuberculose pulmonar | pt_BR |
dc.subject | Reação em cadeia da polimerase | pt_BR |
dc.subject | HIV | pt_BR |
dc.title | Comparison of two laboratory-developed PCR methods for the diagnosis of pulmonary tuberculosis in Brazilian patients with and without HIV infection | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 000782096 | pt_BR |
dc.type.origin | Estrangeiro | pt_BR |
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