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dc.contributor.authorSant'Anna, Fernando Hayashipt_BR
dc.contributor.authorAndrade, Dieime de Souzapt_BR
dc.contributor.authorTrentini, Débora Brochpt_BR
dc.contributor.authorWeber, Shana de Soutopt_BR
dc.contributor.authorSchrank, Irene Silveirapt_BR
dc.date.accessioned2011-07-28T06:01:01Zpt_BR
dc.date.issued2011pt_BR
dc.identifier.issn1471-2091pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/30414pt_BR
dc.description.abstractBackground: Azospirillum amazonense has potential to be used as agricultural inoculant since it promotes plant growth without causing pollution, unlike industrial fertilizers. Owing to this fact, the study of this species has gained interest. However, a detailed understanding of its genetics and physiology is limited by the absence of appropriate genetic tools for the study of this species. Results: Conjugation and electrotransformation methods were established utilizing vectors with broad hostreplication origins (pVS1 and pBBR1). Two genes of interest - glnK and glnB, encoding PII regulatory proteins - were isolated. Furthermore, glnK-specific A. amazonense mutants were generated utilizing the pK19MOBSACB vector system. Finally, a promoter analysis protocol based on fluorescent protein expression was optimized to aid genetic regulation studies on this bacterium. Conclusion: In this work, genetic tools that can support the study of A. amazonense were described. These methods could provide a better understanding of the genetic mechanisms of this species that underlie its plant growth promotion.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofBMC Microbiology. London. Vol. 11, no. 107 (May 2011), p. 1-11pt_BR
dc.rightsOpen Accessen
dc.subjectAzospirillum amazonensept_BR
dc.titleTools for genetic manipulation of the plant growth-promoting bacterium Azospirillum amazonensept_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000780411pt_BR
dc.type.originEstrangeiropt_BR


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