Pollen diet diversity across bee lineages varies with lifestyle rather than colony size

Abstract

The shift to a pollen diet and the evolution of more highly organized societies, i.e., eusocial, were key milestones in bee diversifcation over their evolutionary history, culminating in a high dependence on feeding broods with a large variety of foral resources. Here, we hypothesized that obligatory eusocial bees have a wider diet diver- sity than their relatives with solitary lifestyles, and this would be related to colony size. To test both hypotheses, we surveyed diet breadth data (palynological analysis) based on the Shannon–Wiener index (Hʹ) for 85 bee taxa. We also obtained colony size for 47 eusocial bee species. These data were examined using phylogenetic comparative methods. The results support the generalist strategy as a derived trait for the bee taxa evaluated here. The dietary diversity of eusocial bees (Hʹ: 2.1, on average) was 67.5% higher than that of noneusocial bees (Hʹ: 1.21, on average). There was, however, no relationship between diet breadth and colony size, indicating that smaller colonies can harvest a pollen variety as diverse as larger colonies. Taken together, these results provide new insights into the impact of lifestyle on the diversity of collected pollen. Furthermore, this work sheds light on an advantage of living in more highly structured societies irrespective of the size of the colony.