Microbial leakage in root-end fillings materials after retro-preparation by two ultrasonic tips

Abstract

Aim: the purpose of this study was to evaluate the microbial leakage of root- end fillings materials (ProRoot, MTA- Angelus and Portland cement) after re- tro-preparation by two ultrasonic tips (Osada and CVD). Methodology: seventy maxillary ante- rior human teeth had their root canals prepared using crown-down technique. The apical 3 mm was resected and the root-end cavity was prepared. The sam- ples then were randomly distributed in 6 groups and two controls groups. In order to make the fixation of the teeth, a platform was used, containing the up- per chamber and the lower chamber with Brain Heart Infusion broth (BHI). Approximately 3 mm of the apical part of the root were stored in a BHI cultu- re medium. The samples were inocula- ted with 0.1 ml of the microbial su- spension (E. faecalis + S. aureus + P. ae- ruginosa + B. subtilis + C. albicans) for 60 days. Results: all groups showed a certain de- gree of microbial leakage. There were no statistically significant differences among the experimental groups, both in relation to the retrofilling materials and ultrasonic tips. Conclusion: the present experiment supports previous information from studies about the acceptable sealing quality of MTA and Portland cement when used in root-end fillings.