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dc.contributor.authorHaag, Karen Luisapt_BR
dc.contributor.authorGottstein, Brunopt_BR
dc.contributor.authorMuller, Norbertpt_BR
dc.contributor.authorSchnorr, Andrépt_BR
dc.contributor.authorAyala, Francisco J.pt_BR
dc.date.accessioned2011-01-21T05:59:02Zpt_BR
dc.date.issued2006pt_BR
dc.identifier.issn0031-1820pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/27460pt_BR
dc.description.abstractNumerous genetic variants of the Echinococcus antigen B (AgB) are encountered within a single metacestode. This could be a reflection of gene redundancy or the result of a somatic hypermutation process. We evaluate the complexity of the AgB multigene family by characterizing the upstream promoter regions of the 4 already known genes (EgAgB1-EgAgB4) and evaluating their redundancy in the genome of 3 Echinococcus species (E. granulosus, E. ortleppi and E. multilocularis) using PCR-based approaches. We have ascertained that the number of AgB gene copies is quite variable, both within and between species. The most repetitive gene seems to be AgB3, of which there are more than 110 copies in E. ortleppi. For E. granulosus, we have cloned and characterized 10 distinct upstream promoter regions of AgB3 from a single metacestode. Our sequences suggest that AgB1 and AgB3 are involved in gene conversion. These results are discussed in light of the role of gene redundancy and recombination in parasite evasion mechanisms of host immunity, which at present are known for protozoan organisms, but virtually unknown for multicellular parasites.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofParasitology. Cambridge, Inglaterra. Vol. 133, n. 4 (Oct. 2006), p. 411-419pt_BR
dc.rightsOpen Accessen
dc.subjectEchinococcuspt_BR
dc.subjectHydatid diseaseen
dc.subjectAntigen Ben
dc.subjectHidatidose : Antigenos : Imunodiagnosticopt_BR
dc.subjectAntígeno Bpt_BR
dc.subjectPromoteren
dc.subjectRecombinationen
dc.subjectAntigen diversityen
dc.subjectReal-time PCRen
dc.titleRedundancy and recombination in the Echinococcus AgB multigene family : is there any similarity with protozoan contigency genes?pt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000588215pt_BR
dc.type.originEstrangeiropt_BR


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