Main degradation products of dabigatran etexilate evaluated by LC-UV and LC-ESI-MS, degradation kinetics and in vitro cytotoxicity studies
dc.contributor.author | Bernardi, Raquel Martini | pt_BR |
dc.contributor.author | D'Avila, Felipe Bianchini | pt_BR |
dc.contributor.author | Todeschini, Vítor | pt_BR |
dc.contributor.author | Fasolo, Juliana Maria de Mello Andrade | pt_BR |
dc.contributor.author | Froehlich, Pedro Eduardo | pt_BR |
dc.contributor.author | Bergold, Ana Maria | pt_BR |
dc.date.accessioned | 2021-09-24T04:21:57Z | pt_BR |
dc.date.issued | 2015 | pt_BR |
dc.identifier.issn | 0103-5053 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/230149 | pt_BR |
dc.description.abstract | The present study reports the stability profile of an antithrombotic drug: dabigatran etexilate (DAB). The drug was subjected to thermal degradation at 60 °C and products formed were investigated by liquid chromatography-UV (LC-UV) and liquid chromatography-mass spectrometry (LC-ESI-MS). Chromatographic separation of the degradation products was performed on a GL Sciences Inc. Inertsil ODS-2 column (250 mm × 4.6 mm i.d., with a particle size of 5 µm and pore size of 110 Å) with mobile phase consisting of acetonitrile and ammonium acetate buffer (pH 5.5; 10 mmol L−1 ) (65:35, v/v) pumped at 1.0 mL min−1 flow rate. Column temperature was set at 30 °C and detection at 225 nm using a UV detector. LC-UV method previously validated was extended to LC-ESI-MS for the characterization of the degradation products (DP-01 and DP-02) formed, without complicated isolation or purification processes, based on retention times and confirmation of molecular weight. Degradation kinetics of DAB was also evaluated and could be described as a first-order process (R2 = 0.9900). Furthermore, no evidence of cytotoxicity in human mononuclear cells was observed for DAB degraded samples. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Journal of the Brazilian Chemical Society. Vol. 26, no. 4 (2015), p. 660-666 | pt_BR |
dc.rights | Open Access | en |
dc.subject | Citotoxicidade | pt_BR |
dc.subject | Cytotoxity | en |
dc.subject | Dabigatran etexilate | en |
dc.subject | Cinética de degradação | pt_BR |
dc.subject | Produtos de degradação | pt_BR |
dc.subject | Degradation kinetics | en |
dc.subject | Degradation products | en |
dc.subject | LC-ESI-MS | en |
dc.title | Main degradation products of dabigatran etexilate evaluated by LC-UV and LC-ESI-MS, degradation kinetics and in vitro cytotoxicity studies | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 001018874 | pt_BR |
dc.type.origin | Nacional | pt_BR |
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