OsMTP11 is localised at the Golgi and contributes to Mn tolerance
dc.contributor.author | Farthing, Emily | pt_BR |
dc.contributor.author | Menguer, Paloma Koprovski | pt_BR |
dc.contributor.author | Fett, Janette Palma | pt_BR |
dc.contributor.author | Williams, Lorraine E. | pt_BR |
dc.date.accessioned | 2021-08-11T04:47:40Z | pt_BR |
dc.date.issued | 2017 | pt_BR |
dc.identifier.issn | 2045-2322 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/225527 | pt_BR |
dc.description.abstract | Membrane transporters play a key role in obtaining sufficient quantities of manganese (Mn) but also in protecting against Mn toxicity. We have characterized OsMTP11, a member of the Cation Diffusion Facilitator/Metal Tolerance Protein (CDF/MTP) family of metal cation transporters in Oryza sativa. We demonstrate that OsMTP11 functions in alleviating Mn toxicity as its expression can rescue the Mn-sensitive phenotype of the Arabidopsis mtp11-3 knockout mutant. When expressed stably in Arabidopsis and transiently in rice and tobacco, it localises to the Golgi. OsMTP11 partially rescues the Mn-hypersensitivity of the pmr1 yeast mutant but only slightly alleviates the Zn sensitivity of the zrc1 cot1 yeast mutant. Overall, these results suggest that OsMTP11 predominantly functions as a Mn-transporting CDF with lower affinity for Zn. Site-directed mutagenesis studies revealed four substitutions in OsMTP11 that appear to alter its transport activity. OsMTP11 harbouring a substitution of leucine 150 to a serine fully rescued pmr1 Mn-sensitivity at all concentrations tested. The other substitutions, including those at conserved DxxxD domains, reduced complementation of pmr1 to different levels. This indicates their importance for OsMTP11 function and is a starting point for refining transporter activity/specificity. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Scientific reports. London. Vol. 7, (Nov. 2017), article 15258, p. 1-13 | pt_BR |
dc.rights | Open Access | en |
dc.subject | Oryza sativa | pt_BR |
dc.subject | Arabidopsis | pt_BR |
dc.title | OsMTP11 is localised at the Golgi and contributes to Mn tolerance | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 001055118 | pt_BR |
dc.type.origin | Estrangeiro | pt_BR |
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