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dc.contributor.authorLopes, Tiago Falcónpt_BR
dc.contributor.authorFoletto, Kelly Carraropt_BR
dc.contributor.authorSiebert, Marinapt_BR
dc.contributor.authorPinto, Denise Entrudopt_BR
dc.contributor.authorAndrades, Michael Evertonpt_BR
dc.contributor.authorBertoluci, Marcello Casacciapt_BR
dc.date.accessioned2020-11-14T04:22:42Zpt_BR
dc.date.issued2020pt_BR
dc.identifier.issn1415-4757pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/215040pt_BR
dc.description.abstractThe effects of non-nutritive sweeteners (NNS) on the gut microbiota are an area of increasing research interest due to their potential influence on weight gain, insulin resistance, and inflammation. Studies have shown that mice and rats fed saccharin develop weight gain and metabolic alterations, possibly related to changes in gut microbiota. Here, we hypothesized that chronic exposure to a commercial NNS would change the gut microbiota composition in Wistar rats when compared to sucrose exposure. To test this hypothesis, Wistar rats were fed either NNS- or sucrose-supplemented yogurt for 17 weeks alongside standard chow (ad libitum). The gut microbiome was assessed by 16S rDNA deep sequencing. Assembly and quantification were conducted using the Brazilian Microbiome Project pipeline for Ion Torrent data with modifications. Statistical analyses were performed in the R software environment. We found that chronic feeding of a commercial NNS-sweetened yogurt to Wistar rats, within the recommended dose range, did not significantly modify gut microbiota composition in comparison to sucrose-sweetened yogurt. Our findings do not support the hypothesis that moderate exposure to NNS is associated with changes in gut microbiota pattern compared to sucrose, at least in this experimental model.1en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofGenetics and molecular biology. Ribeirão Preto. Vol. 43, n.1 (2020), e20190028,6 p.pt_BR
dc.rightsOpen Accessen
dc.subject6S rDNAen
dc.subjectSequenciamento de nucleotídeos em larga escalapt_BR
dc.subjectDeep sequencingen
dc.subjectIogurtept_BR
dc.subjectSaccharinen
dc.subjectSacarosept_BR
dc.subjectSacarinapt_BR
dc.subjectCyclamateen
dc.subjectMicrobiotapt_BR
dc.subjectYogurten
dc.subjectModelos animaispt_BR
dc.titleMetabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar ratspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001117871pt_BR
dc.type.originNacionalpt_BR


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