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dc.contributor.authorGutierrez, Lucila Ludmila Paulapt_BR
dc.contributor.authorMazzotti, Nicolle Gollopt_BR
dc.contributor.authorAraújo, Alex Sander da Rosapt_BR
dc.contributor.authorKlipel, Rodrigo Blauthpt_BR
dc.contributor.authorBelló-Klein, Adrianept_BR
dc.contributor.authorPiedras, Tania Regina Gattelli Fernandespt_BR
dc.contributor.authorLlesuy, Susana Franciscapt_BR
dc.date.accessioned2010-04-24T04:15:44Zpt_BR
dc.date.issued2006pt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/21210pt_BR
dc.description.abstractThe present study was designed to evaluate the time course changes in peripheral markers of oxidative stress in a chronic HgCl2 intoxication model. Twenty male adult Wistar rats were treated subcutaneously daily for 30 days and divided into two groups of 10 animals each: Hg, which received HgCl2 (0.16 mg kg-1 day-1), and control, receiving the same volume of saline solution. Blood was collected at the first, second and fourth weeks of Hg administration to evaluate lipid peroxidation (LPO), total radical trapping antioxidant potential (TRAP), and superoxide dismutase (Cu,Zn-SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and catalase (CAT). HgCl2 administration induced a rise (by 26%) in LPO compared to control (143 ± 10 cps/mg hemoglobin) in the second week and no difference was found at the end of the treatment. At that time, GST and GPx were higher (14 and 24%, respectively) in the Hg group, and Cu,Zn-SOD was lower (54%) compared to control. At the end of the treatment, Cu,Zn-SOD and CAT were higher (43 and 10%, respectively) in the Hg group compared to control (4.6 ± 0.3 U/mg protein; 37 ± 0.9 pmol/mg protein, respectively). TRAP was lower (69%) in the first week compared to control (43.8 ± 1.9 mM Trolox). These data provide evidence that HgCl2 administration is accompanied by systemic oxidative damage in the initial phase of the process, which leads to adaptive changes in the antioxidant reserve, thus decreasing the oxidative injury at the end of 30 days of HgCl2 administration. These results suggest that a preventive treatment with antioxidants would help to avoid oxidative damage in subjects with chronic intoxication.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 39, n. 6 (jun. 2006), p. 767-772pt_BR
dc.rightsOpen Accessen
dc.subjectEstresse oxidativopt_BR
dc.subjectHeavy metalsen
dc.subjectAntioxidantespt_BR
dc.subjectAntioxidant enzymesen
dc.subjectLipid peroxidationen
dc.subjectTotal radical-trapping antioxidant potentialen
dc.subjectHgCl2en
dc.titlePeripheral markers of oxidative stress in chronic mercuric chloride intoxicationpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000609138pt_BR
dc.type.originNacionalpt_BR


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