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dc.contributor.authorPassos, Eduardo Pandolfipt_BR
dc.contributor.authorBrugnara, Laurapt_BR
dc.contributor.authorFacin, Andrea Cintrapt_BR
dc.contributor.authorRiffel, Alessandropt_BR
dc.contributor.authorLima, Geraldo Rodrigues dept_BR
dc.contributor.authorFreitas, V.pt_BR
dc.contributor.authorBrandelli, Adrianopt_BR
dc.date.accessioned2010-04-24T04:15:31Zpt_BR
dc.date.issued1999pt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/21153pt_BR
dc.description.abstractThe effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophoretreated sperm showed that NGP-induced exocytosis differed significantly between the PFc (11%) and PFe/PFi groups (17%), and the ionophore-induced AR was higher for PFi (33%) than PFc/PFe (25%). The incidence of the NGP-induced AR was reduced in the first hour of pre-incubation with PFc and remained nearly constant throughout 4 h of incubation. The present data indicate that PF possesses a protective factor which prevents premature AR.en
dc.format.mimetypeapplication/pdf
dc.language.isoporpt_BR
dc.relation.ispartofBrazilian journal of medical and biological research. Ribeirão Preto, SP. Vol. 32, no. 1 (Jan. 1999), p. 59-65pt_BR
dc.rightsOpen Accessen
dc.subjectEndometriosept_BR
dc.subjectAcrosome reactionen
dc.subjectN-acetilglucosaminidasept_BR
dc.subjectEndometriosisen
dc.subjectInfertilityen
dc.subjectReação acrossômicapt_BR
dc.subjectInfertilidadept_BR
dc.subjectPeritoneal fluiden
dc.subjectFluido peritonealpt_BR
dc.subjectSpermen
dc.subjectExocitosept_BR
dc.titlePeritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neolycoproteinpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000294542pt_BR
dc.type.originNacionalpt_BR


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