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dc.contributor.authorBrandão, Vanessa Duarte Martinspt_BR
dc.contributor.authorManfredini, Vanusapt_BR
dc.contributor.authorBenfato, Mara da Silveirapt_BR
dc.date.accessioned2010-04-16T09:13:19Zpt_BR
dc.date.issued2005pt_BR
dc.identifier.issn1517-8382pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/20160pt_BR
dc.description.abstractSaccharomyces cerevisiae mutants deficient in superoxide dismutase genes (sod1Δ, sod2Δ and sod1Δsod2Δ mutants) in a stationary phase of growth under high aeration conditions were subjected to H2O2 stress. All the mutants were sensitive after H2O2 treatment. Glutathione peroxidase levels were significantly lower in sod1Δ and sod2Δ single mutants than in the wild-type without treatment. After exposure to H2O2 concentrations, glutathione peroxidase levels were increased in sod1Δsod2Δ double mutants and the sod2Δ single mutant, while sod1Δ maintained lower gluthatione peroxidase activities. The sod2Δ mutant demonstrated a similar catalase activity to that of the wild-type without treatment, whilst decreased catalase activity was observed in conditions of low aeration. Untreated sod1Δsod2Δ double mutant cells presented a lower catalase activity. Catalase levels were higher under high aeration conditions than under microaerophilic conditions, including in sod1Δsod2Δ cells that contain less H2O2, since SOD catalyzes the cleavage of superoxide producing H2O2 and oxygen. We suggest that catalase is not essential for sod mutants under normal conditions, but plays an important role in the acquisition of tolerance to oxidative stress induced by high aeration.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of microbiology. São Paulo, SP. Vol. 36, n. 4, (out./dez. 2005), p. 347-351pt_BR
dc.rightsOpen Accessen
dc.subjectCatalaseen
dc.subjectCatalasept_BR
dc.subjectSaccharomyces cerevisiaept_BR
dc.subjectSuperoxide dismutaseen
dc.subjectSuperóxido dismutasept_BR
dc.subjectHigh aerationen
dc.subjectAeraçãopt_BR
dc.subjectHydrogen peroxideen
dc.subjectS. cerevisiaeen
dc.subjectPeróxido de hidrogêniopt_BR
dc.titleHigh levels of catalase in SOD mutants of Saccharomyces cerevisae in high aeration conditionspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000521604pt_BR
dc.type.originNacionalpt_BR


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