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dc.contributor.authorPancotto, Lisiane Rechpt_BR
dc.contributor.authorNodari, Carolina Silvapt_BR
dc.contributor.authorRozales, Franciéli Pedrottipt_BR
dc.contributor.authorSoldi, Tatiane Cristinapt_BR
dc.contributor.authorSiqueira, Carolina Gomespt_BR
dc.contributor.authorFreitas, Ana Lucia Peixoto dept_BR
dc.contributor.authorBarth, Afonso Luispt_BR
dc.date.accessioned2019-11-02T03:51:31Zpt_BR
dc.date.issued2018pt_BR
dc.identifier.issn1517-8382pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/201253pt_BR
dc.description.abstractThe global emergence of carbapenemases led to the need of developing new methods for their rapid detection. The aim of this study was to evaluate the performance of the rapid tests for carbapenemase-producing and non-producing Enterobacteriaceae. Carbapenem non-susceptible Enterobacteriaceae from a surveillance study submitted to a multiplex real time PCR for carbapenemase detection were included in this study. The isolates were subjected to the rapid phenotypic tests Carba NP, Blue-Carba and Carbapenem Inactivation Method (CIM). A total of 83 carbapenemase-producing (43) and non-producing (40) isolates were included in the study. The sensitivity/specificity were 62.7%/97.5%, 95.3%/100%, and 74.4%/97.5% for Carba NP, Blue-Carba and CIM, respectively. Both Carba NP and Blue-Carba presented their final results after 75 min of incubation; the final results for CIM were obtained only after 8 h. Failure to detect OXA-370 carbapenemase was the main problem for Carba NP and CIM assays. As the Blue-Carba presented the highest sensitivity, it can be considered the best screening test. Conversely, CIM might be the easiest to perform, as it does not require special reagents. The early detection of carbapenemases aids to establish infection control measures and prevent carbapenemases to spread reducing the risk of healthcare associated infections and therapeutic failure.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of microbiology. Vol. 49, no. 4 (Oct./Dec. 2018), p. 914-918pt_BR
dc.rightsOpen Accessen
dc.subjectEnterobacteriaceaept_BR
dc.subjectCarbapenemasesen
dc.subjectEnterobacteriáceas resistentes a carbapenêmicospt_BR
dc.subjectRapid testsen
dc.subjectCarba NPen
dc.subjectBlue-Carbaen
dc.subjectCIMen
dc.titlePerformance of rapid tests for carbapenemase detection among Brazilian Enterobacteriaceae isolatespt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001103994pt_BR
dc.type.originNacionalpt_BR


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