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dc.contributor.authorGabiatti, Gémersonpt_BR
dc.contributor.authorGrezzana Filho, Tomáz de Jesus Mariapt_BR
dc.contributor.authorCerski, Carlos Thadeu Schmidtpt_BR
dc.contributor.authorBofill, Carlos Medeirospt_BR
dc.contributor.authorValle, Stella de Fariapt_BR
dc.contributor.authorCorso, Carlos Otaviopt_BR
dc.date.accessioned2019-10-17T03:52:03Zpt_BR
dc.date.issued2018pt_BR
dc.identifier.issn0102-8650pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/200777pt_BR
dc.description.abstractPurpose: To develop a new 24 hour extended liver ischemia and reperfusion (LIR) model analyzing the late biochemical and histopathological results of the isolated and combined application of recognized hepatoprotective mechanisms. In addition, we used a new stratification with zoning to classify the histological lesion. Methods: A modified animal model of severe hepatic damage produced through 90 minutes of segmental ischemia (70% of the organ) and posterior observation for 24 hours of reperfusion, submitted to ischemic preconditioning (IPC) and topical hypothermia (TH) at 26ºC, in isolation or in combination, during the procedure. Data from intraoperative biometric parameters, besides of late biochemical markers and histopathological findings, both at 24 hours evolution time, were compared with control (C) and normothermic ischemia (NI) groups. Results: All groups were homogeneous with respect to intraoperative physiological parameters. There were no losses once the model was stablished. Animals subjected to NI and IPC had worse biochemical (gamma-glutamyl transpeptidase, alkaline phosphatase, lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, direct bilirubin, and total bilirubin) and histopathological scores (modified Suzuki score) compared to those of control groups and groups with isolated or associated TH (p < 0.05). Conclusion: The new extended model demonstrates liver ischemia and reperfusion at 24 hour of evolution and, in this extreme scenario, only the groups subjected to topical hypothermia, combined with ischemic preconditioning or alone, had better outcomes than those subjected to only ischemic preconditioning and normothermic ischemia, reaching similar biochemical and histopathological scores to those of the control group.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofActa cirúrgica brasileira. Vol. 33, no. 10 (2018), p. 924-934pt_BR
dc.rightsOpen Accessen
dc.subjectIsquemiapt_BR
dc.subjectIschemic preconditioningen
dc.subjectIschemiaen
dc.subjectHipotermiapt_BR
dc.subjectReperfusionen
dc.subjectRatospt_BR
dc.subjectLiveren
dc.subjectModelos animaispt_BR
dc.subjectHypothermiaen
dc.subjectReperfusãopt_BR
dc.subjectRatsen
dc.subjectFígadopt_BR
dc.subjectPrecondicionamento isquêmicopt_BR
dc.titleTopical hepatic hypothermia associated with ischemic preconditioning : histopathological and biochemical analysis of ischemia reperfusion damage in a 24 hour modept_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001104484pt_BR
dc.type.originNacionalpt_BR


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