Cytotoxicity and anti-Sporothrix brasiliensis activity of the Origanum majorana Linn. oil
dc.contributor.author | Waller, Stefanie Bressan | pt_BR |
dc.contributor.author | Madrid, Isabel Martins | pt_BR |
dc.contributor.author | Ferraz, Vany Perpetua | pt_BR |
dc.contributor.author | Picoli, Tony | pt_BR |
dc.contributor.author | Cleff, Marlete Brum | pt_BR |
dc.contributor.author | Faria, Renata Osório de | pt_BR |
dc.contributor.author | Meireles, Mário Carlos Araújo | pt_BR |
dc.contributor.author | Mello, Joao Roberto Braga de | pt_BR |
dc.date.accessioned | 2019-10-17T03:51:42Z | pt_BR |
dc.date.issued | 2016 | pt_BR |
dc.identifier.issn | 1517-8382 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/200763 | pt_BR |
dc.description.abstract | The study aimed to evaluate the anti-Sporothrix sp. activity of the essential oil of Origanummajorana Linn. (marjoram), its chemical analysis, and its cytotoxic activity. A total of 18fungal isolates of Sporothrix brasiliensis (n: 17) from humans, dogs and cats, and a standardstrain of Sporothrix schenckii (n: 1) were tested using the broth microdilution technique (Clin-ical and Laboratory Standard Institute – CLSI M27-A3) and the results were expressed inminimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC). TheMIC50and MIC90of itraconazole against S. brasiliensis were 2 g/mL and 8 g/mL, respectively,and the MFC50and MFC90were 2 g/mL and >16 g/mL, respectively, with three S. brasilien-sis isolates resistant to antifungal. S. schenckii was sensitive at MIC of 1 g/mL and MFC of8 g/mL. For the oil of O. majorana L., all isolates were susceptible to MIC of ≤2.25–9 mg/mLand MFC of ≤2.25–18 mg/mL. The MIC50and MIC90were ≤2.25 mg/mL and 4.5 mg/mL, respec-tively, and the MFC50/90values were twice more than the MIC. Twenty-two compounds wereidentified by gas chromatography with a flame ionization detector (CG-FID) and 1,8-cineoleand 4-terpineol were the majority. Through the colorimetric (MTT) assay, the toxicity wasobserved in 70–80% of VERO cells between 0.078 and 5 mg/mL. For the first time, the studydemonstrated the satisfactory in vitro anti-Sporothrix sp. activity of marjoram oil and furtherstudies are needed to ensure its safe and effective use. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Brazilian journal of microbiology. Vol. 47, no. 4 (Oct./Dec. 2016), p. 896-901 | pt_BR |
dc.rights | Open Access | en |
dc.subject | Esporotricose | pt_BR |
dc.subject | Sporothrix | pt_BR |
dc.subject | Origanum | pt_BR |
dc.subject | Farmacorresistência fúngica | pt_BR |
dc.title | Cytotoxicity and anti-Sporothrix brasiliensis activity of the Origanum majorana Linn. oil | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 001103339 | pt_BR |
dc.type.origin | Nacional | pt_BR |
Este item está licenciado na Creative Commons License
-
Artigos de Periódicos (40021)Ciências Biológicas (3157)