An analysis of the global expression of microRNAs in an experimental model of physiological left ventricular hypertrophy
dc.contributor.author | Martinelli, Nidiane Carla | pt_BR |
dc.contributor.author | Cohen, Carolina Rodrigues | pt_BR |
dc.contributor.author | Santos, Kátia Gonçalves dos | pt_BR |
dc.contributor.author | Castro, Mauro A. | pt_BR |
dc.contributor.author | Biolo, Andreia | pt_BR |
dc.contributor.author | Frick, Luzia Menegotto | pt_BR |
dc.contributor.author | Ferreira, Daiane Nicoli Silvello dos Santos | pt_BR |
dc.contributor.author | Lopes, Amanda | pt_BR |
dc.contributor.author | Schneider, Stéfanie Ingrid dos Reis | pt_BR |
dc.contributor.author | Andrades, Michael Everton | pt_BR |
dc.contributor.author | Clausell, Nadine Oliveira | pt_BR |
dc.contributor.author | Matte, Ursula da Silveira | pt_BR |
dc.contributor.author | Rohde, Luis Eduardo Paim | pt_BR |
dc.date.accessioned | 2019-09-07T02:33:12Z | pt_BR |
dc.date.issued | 2014 | pt_BR |
dc.identifier.issn | 1932-6203 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/198916 | pt_BR |
dc.description.abstract | Background: MicroRNAs (miRs) are a class of small non-coding RNAs that regulate gene expression. Studies of transgenic mouse models have indicated that deregulation of a single miR can induce pathological cardiac hypertrophy and cardiac failure. The roles of miRs in the genesis of physiological left ventricular hypertrophy (LVH), however, are not well understood. Objective: To evaluate the global miR expression in an experimental model of exercise-induced LVH. Methods: Male Balb/c mice were divided into sedentary (SED) and exercise (EXE) groups. Voluntary exercise was performed on an odometer-monitored metal wheels for 35 days. Various tests were performed after 7 and 35 days of training, including a transthoracic echocardiography, a maximal exercise test, a miR microarray (miRBase v.16) and qRT-PCR analysis. Results: The ratio between the left ventricular weight and body weight was increased by 7% in the EXE group at day 7 (p, 0.01) and by 11% at day 35 of training (p,0.001). After 7 days of training, the microarray identified 35 miRs that were differentially expressed between the two groups: 20 were up-regulated and 15 were down-regulated in the EXE group compared with the SED group (p = 0.01). At day 35 of training, 25 miRs were differentially expressed: 15 were up-regulated and 10 were decreased in the EXE animals compared with the SED animals (p,0.01). The qRT-PCR analysis demonstrated an increase in miR-150 levels after 35 days and a decrease in miR-26b, miR-27a and miR-143 after 7 days of voluntary exercise. Conclusions: We have identified new miRs that can modulate physiological cardiac hypertrophy, particularly miR-26b, -150, -27a and -143. Our data also indicate that previously established regulatory gene pathways involved in pathological LVH are not changed in physiological LVH. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | PLoS ONE. San Francisco. Vol. 9, no. 4 (Apr. 2014), e93271, 10 p. | pt_BR |
dc.rights | Open Access | en |
dc.subject | MicroRNAs | pt_BR |
dc.subject | Hipertrofia ventricular esquerda | pt_BR |
dc.title | An analysis of the global expression of microRNAs in an experimental model of physiological left ventricular hypertrophy | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 000943367 | pt_BR |
dc.type.origin | Estrangeiro | pt_BR |
Este item está licenciado na Creative Commons License
-
Artigos de Periódicos (39774)Ciências da Saúde (10633)