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dc.contributor.authorThéry, Clotildept_BR
dc.contributor.authorZuba-Surma, Ewa K.pt_BR
dc.contributor.authorVeit, Tiago Deganipt_BR
dc.date.accessioned2018-12-19T04:07:18Zpt_BR
dc.date.issued2018pt_BR
dc.identifier.issn2001-3078pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/187209pt_BR
dc.description.abstractThe last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, andmany other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofJournal of Extracellular Vesicles. Järfälla, Sweden. Vol. 8, no.1, (2018), 1535750pt_BR
dc.rightsOpen Accessen
dc.subjectExtracellular vesiclesen
dc.subjectVesículas extracelularespt_BR
dc.subjectExossomospt_BR
dc.subjectExosomesen
dc.subjectEctosomesen
dc.subjectMicropartículas derivadas de célulaspt_BR
dc.subjectMicrovesiclesen
dc.subjectMinimal information requirementsen
dc.subjectGuidelinesen
dc.subjectStandardizationen
dc.subjectMicroparticlesen
dc.subjectRigoren
dc.subjectReproducibilityen
dc.titleMinimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelinespt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001082698pt_BR
dc.type.originEstrangeiropt_BR


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