Accumulation of the antioxidant indole alkaloid brachycerine under high temperature and high irradiance in leaf disks of Psychotria brachyceras
dc.contributor.advisor | Fett Neto, Arthur Germano | pt_BR |
dc.contributor.author | Tasca, Ramsés Assul Jessé Cantelli | pt_BR |
dc.date.accessioned | 2018-10-18T02:42:26Z | pt_BR |
dc.date.issued | 2014 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/183531 | pt_BR |
dc.description.abstract | Psychotria brachyceras Müll.Arg. (Rubiaceae) is an understorey shrub of the Atlantic Forest whose leaves accumulate brachycerine, an antioxidant monoterpene indole alkaloid (MIA). This work aimed at evaluating the response of this MIA in leaf disks upon exposure to different temperatures and irradiance, similar to those experienced by the plant in its environment. Leaf disks obtained from growth room-acclimated cuttings were submitted to three distinct series of treatments: 1) Light - two days under control condition (50 μmol.m-2.s-1) in a growth chamber followed by three days treatment of exposure to high irradiance (500 μmol.m-2.s-1); 2) Temperature Cold/Heat – three days under control conditions (25ºC) and then exposure for two days to 10°C or 40°C; 3) Acclimation – gradual heat increase from 25°C up to 40°C. Brachycerine, chlorophyll, and lipid peroxidation levels were not significantly affected by high light or cold exposure, although a decrease in chlorophyll a/b ratio was observed under high irradiance. In contrast, there was increased accumulation of brachycerine at elevated temperatures (where higher cell damage was expected) and significantly lower lipid peroxidation, indicating that this MIA is possibly playing a central role in preventing or mitigating cell damage. A threshold temperature to trigger alkaloid accumulation was identified at approximately 40ºC. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | por | pt_BR |
dc.rights | Open Access | en |
dc.subject | Temperature stress | en |
dc.subject | Psychotria brachyceras | pt_BR |
dc.subject | Braquicerina | pt_BR |
dc.subject | Irradiance stress | en |
dc.subject | Estresse oxidativo | pt_BR |
dc.subject | MIA | en |
dc.subject | ROS | en |
dc.subject | Psychotria | en |
dc.subject | Brachycerine | en |
dc.title | Accumulation of the antioxidant indole alkaloid brachycerine under high temperature and high irradiance in leaf disks of Psychotria brachyceras | pt_BR |
dc.type | Trabalho de conclusão de graduação | pt_BR |
dc.contributor.advisor-co | Matsuura, Hélio Nitta | pt_BR |
dc.identifier.nrb | 000949824 | pt_BR |
dc.degree.grantor | Universidade Federal do Rio Grande do Sul | pt_BR |
dc.degree.department | Instituto de Biociências | pt_BR |
dc.degree.local | Porto Alegre, BR-RS | pt_BR |
dc.degree.date | 2014 | pt_BR |
dc.degree.graduation | Ciências Biológicas: Bacharelado | pt_BR |
dc.degree.level | graduação | pt_BR |
Este item está licenciado na Creative Commons License
-
TCC Ciências Biológicas (1289)