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dc.contributor.authorLenherr, Sara Mariept_BR
dc.contributor.authorTsai, Sheaumeipt_BR
dc.contributor.authorSilva Neto, Brasilpt_BR
dc.contributor.authorSullivan, Travis B.pt_BR
dc.contributor.authorCimmino, Cara B.pt_BR
dc.contributor.authorLogvinenko, Tanyapt_BR
dc.contributor.authorGee, Jasonpt_BR
dc.contributor.authorHuang, Weipt_BR
dc.contributor.authorLibertino, John A.pt_BR
dc.contributor.authorSummerhayes, Ian C.pt_BR
dc.contributor.authorRieger-Christ, Kimberly M.pt_BR
dc.date.accessioned2018-08-18T03:01:15Zpt_BR
dc.date.issued2017pt_BR
dc.identifier.issn2073-4425pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/181117pt_BR
dc.description.abstractAbstract: The objective of this study was to identify a panel of microRNAs (miRNAs) differentially expressed in high-grade non-muscle invasive (NMI; TaG3–T1G3) urothelial carcinoma that progress to muscle-invasive disease compared to those that remain non-muscle invasive, whether recurrence happens or not. Eighty-nine high-grade NMI urothelial carcinoma lesions were identified and total RNA was extracted from paraffin-embedded tissue. Patients were categorized as either having a non-muscle invasive lesion with no evidence of progression over a 3-year period or as having a similar lesion showing progression to muscle invasion over the same period. In addition, comparison of miRNA expression levels between patients with and without prior intravesical therapy was performed. Total RNA was pooled for microarray analysis in each group (non-progressors and progressors), and qRT-PCR of individual samples validated differential expression between non-progressive and progressive lesions. MiR-32-5p, -224-5p, and -412-3p were associated with cancer-specific survival. Downregulation of miR-203a-3p and miR-205-5p were significantly linked to progression in non-muscle invasive bladder tumors. These miRNAs include those implicated in epithelial mesenchymal transition, previously identified as members of a panel characterizing transition from the non-invasive to invasive phenotype in bladder tumors. Furthermore, we were able to identify specific miRNAs that are linked to postoperative outcome in patients with high grade NMI urothelial carcinoma of the bladder (UCB) that progressed to muscle-invasive (MI) disease.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofGenes. Basel. Vol. 8, issue 2 (Feb. 2017), 15 p.pt_BR
dc.rightsOpen Accessen
dc.subjectMicroRNAen
dc.subjectMicroRNAspt_BR
dc.subjectNon-muscle invasive bladder canceren
dc.subjectAdministração intravesicalpt_BR
dc.subjectProgressionen
dc.subjectCarcinoma de células de transiçãopt_BR
dc.subjectIntravesical therapy Genes 2017en
dc.subjectNeoplasias da bexiga urináriapt_BR
dc.subjectProgressão da doençapt_BR
dc.subjectFixação de tecidospt_BR
dc.titleMicroRNA expression profile identifies high grade, non-muscle-invasive bladder tumors at elevated risk to progress to an invasive phenotypept_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001072210pt_BR
dc.type.originEstrangeiropt_BR


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