Cofilin-1 levels and intracellular localization are associated with melanoma prognosis in a cohort of patients
dc.contributor.author | Bracalente, Candelaria | pt_BR |
dc.contributor.author | Rinflerch, Adriana | pt_BR |
dc.contributor.author | Ibañez, Irene Laura | pt_BR |
dc.contributor.author | Garcia, Francisco M. | pt_BR |
dc.contributor.author | Volonteri, Victoria | pt_BR |
dc.contributor.author | Galimberti, Gastón N. | pt_BR |
dc.contributor.author | Klamt, Fabio | pt_BR |
dc.contributor.author | Durán, Hebe | pt_BR |
dc.date.accessioned | 2018-06-22T02:29:17Z | pt_BR |
dc.date.issued | 2018 | pt_BR |
dc.identifier.issn | 1949-2553 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/179641 | pt_BR |
dc.description.abstract | Melanoma is an aggressive cancer with highly metastatic ability. We propose cofilin-1, a key protein in the regulation of actin dynamics and migration, as a prognostic marker. We determined cofilin-1 levels in a retrospective cohort of patients with melanomas and benign lesions of melanocytes (nevi) by immunohistochemistry. Higher cofilin-1 levels were found in malignant melanoma (MM) with Breslow Index (BI)>2 vs MM with BI<2, melanoma in situ (MIS) and nevi and also in MM with metastasis vs MM without detected metastasis. Kaplan-Meier survival curves were performed, clustering patients according to either the type of melanocytic lesions or cofilin-1 level. Survival curves demonstrated worse prognosis of patients with high vs low cofilin-1 levels. TCGA database analysis of melanoma also showed low survival in patients with upregulated cofilin-1 mRNA vs patients without alteration in CFL1 mRNA expression. As cofilin-1 has a dual function depending on its intracellular localization, we evaluated nuclear and cytoplasmic levels of cofilin-1 in melanoma and nevi samples by immunofluorescence. MM with high Breslow index and metastatic cells not only presented cytoplasmic cofilin-1, but also showed this protein at the nucleus. An increase in nuclear/cytoplasmic cofilin-1 mean fluorescence ratio was observed in MM with BI>2 vs MM with BI<2, MIS and nevi. In conclusion, an association of cofilin-1 levels with malignant features and an inverse correlation with survival were demonstrated. Moreover, this study suggests that not only the higher levels of cofilin-1, but also its nuclear localization can be proposed as marker of worse outcome of patients with melanoma. | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Oncotarget. Albany. Vol. 9, no. 35 (May 2018), p. 24097-24108 | pt_BR |
dc.rights | Open Access | en |
dc.subject | Cofilina 1 | pt_BR |
dc.subject | Melanoma | pt_BR |
dc.subject | Metástase neoplásica | pt_BR |
dc.subject | Sinais de localização nuclear | pt_BR |
dc.title | Cofilin-1 levels and intracellular localization are associated with melanoma prognosis in a cohort of patients | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 001069662 | pt_BR |
dc.type.origin | Estrangeiro | pt_BR |
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