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dc.contributor.authorXavier, Marina Amaralpt_BR
dc.contributor.authorTirloni, Lucaspt_BR
dc.contributor.authorPinto, Antonio Frederico Michelpt_BR
dc.contributor.authorDiedrich, Jolene K.pt_BR
dc.contributor.authorYates III, John R.pt_BR
dc.contributor.authorMulenga, Albertpt_BR
dc.contributor.authorLogullo, Carlospt_BR
dc.contributor.authorVaz Junior, Itabajara da Silvapt_BR
dc.contributor.authorSeixas, Adrianapt_BR
dc.contributor.authorTermignoni, Carlospt_BR
dc.date.accessioned2018-04-26T02:32:38Zpt_BR
dc.date.issued2018pt_BR
dc.identifier.issn2045-2322pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/175011pt_BR
dc.description.abstractTicks are arthropod ectoparasites of importance for public and veterinary health. The understanding of tick oogenesis and embryogenesis could contribute to the development of novel control methods. However, to date, studies on the temporal dynamics of proteins during ovary development were not reported. In the present study we followed protein profile during ovary maturation. Proteomic analysis of ovary extracts was performed by liquid chromatography-tandem mass spectrometry (LC-MS/ MS) using shotgun strategy, in addition to dimethyl labelling-based protein quantification. A total of 3,756 proteins were identified, which were functionally annotated into 30 categories. Circa 80% of the annotated proteins belong to categories related to basal metabolism, such as protein synthesis and modification machineries, nuclear regulation, cytoskeleton, proteasome machinery, transcriptional machinery, energetic metabolism, extracellular matrix/cell adhesion, immunity, oxidation/ detoxification metabolism, signal transduction, and storage. The abundance of selected proteins involved in yolk uptake and degradation, as well as vitellin accumulation during ovary maturation, was assessed using dimethyl-labelling quantification. In conclusion, proteins identified in this study provide a framework for future studies to elucidate tick development and validate candidate targets for novel control methods.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofScientific reports. London. Vol. 8 (2018), 4698, 14 p.pt_BR
dc.rightsOpen Accessen
dc.subjectRhipicephalus micropluspt_BR
dc.subjectOogenesept_BR
dc.subjectVitelogênesept_BR
dc.subjectEmbriogenesept_BR
dc.subjectProteômicapt_BR
dc.subjectControle biologicopt_BR
dc.titleA proteomic insight into vitellogenesis during tick ovary maturationpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001064303pt_BR
dc.type.originEstrangeiropt_BR


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