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dc.contributor.authorDias, Lucinara Daddapt_BR
dc.contributor.authorCasali, Karina Rabellopt_BR
dc.contributor.authorGhem, Carinept_BR
dc.contributor.authorSilva, Melissa Kristocheck dapt_BR
dc.contributor.authorSausen, Grasielept_BR
dc.contributor.authorPalma, Patricia Vianna Boninipt_BR
dc.contributor.authorCovas, Dimas T.pt_BR
dc.contributor.authorKalil, Renato Abdala Karampt_BR
dc.contributor.authorSchaan, Beatriz D'Agordpt_BR
dc.contributor.authorNardi, Nance Beyerpt_BR
dc.contributor.authorMarkoski, Melissa Medeirospt_BR
dc.date.accessioned2017-11-28T02:29:17Zpt_BR
dc.date.issued2017pt_BR
dc.identifier.issn1479-5876pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/170614pt_BR
dc.description.abstractBackground: In an attempt to increase the therapeutic potential for myocardial regeneration, there is a quest for new cell sources and types for cell therapy protocols. The pathophysiology of heart diseases may affect cellular characteristics and therapeutic results. Methods: To study the proliferative and differentiation potential of mesenchymal stem cells (MSC), isolated from bone marrow (BM) of sternum, we made a comparative analysis between samples of patients with ischemic (IHD) or non-ischemic valvular (VHD) heart diseases. We included patients with IHD (n = 42) or VHD (n = 20), with average age of 60 years and no differences in cardiovascular risk factors. BM samples were collected (16.4 ± 6 mL) and submitted to centrifugation with Ficoll-Paque, yielding 4.5 ± 1.5 × 107 cells/mL. Results: Morphology, immunophenotype and differentiation ability had proven that the cultivated sternal BM cells had MSC features. The colony forming unit-fibroblast (CFU-F) frequency was similar between groups (p = 0.510), but VHD samples showed positive correlation to plated cells vs. CFU-F number (r = 0.499, p = 0.049). The MSC culture was established in 29% of collected samples, achieved passage 9, without significant difference in expansion kinetics between groups (p > 0.05). Dyslipidemia and the use of statins was associated with culture establishment for IHD patients (p = 0.049 and p = 0.006, respectively). Conclusions: Together, these results show that the sternum bone can be used as a source for MSC isolation, and that ischemic or valvular diseases do not influence the cellular yield, culture establishment or in vitro growth kinetics.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofJournal of translational medicine. London. Vol. 15 (May 2017), 161, 11 p.pt_BR
dc.rightsOpen Accessen
dc.subjectMSC establishmenten
dc.subjectDoenças cardiovascularespt_BR
dc.subjectStem cells cultureen
dc.subjectDiabetes mellituspt_BR
dc.subjectMortalidadept_BR
dc.subjectCell therapyen
dc.subjectValvular heart diseaseen
dc.subjectIschemic heart diseaseen
dc.titleMesenchymal stem cells from sternum : the type of heart disease, ischemic or valvular, does not influence the cell culture establishment and growth kineticspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001053379pt_BR
dc.type.originEstrangeiropt_BR


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