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dc.contributor.authorNóbrega, L.M.M.pt_BR
dc.contributor.authorMontagner, Franciscopt_BR
dc.contributor.authorRibeiro, A.C.pt_BR
dc.contributor.authorMayer, Marcia Pinto Alvespt_BR
dc.contributor.authorGomes, Brenda Paula Figueiredo de Almeidapt_BR
dc.date.accessioned2016-08-19T02:16:21Zpt_BR
dc.date.issued2016pt_BR
dc.identifier.issn0103-6440pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/147393pt_BR
dc.description.abstractThe objective of this study was to investigate the bacterial composition present in root canals of teeth associated with acute apical abscess by molecular identification (16S rRNA) of cultivable bacteria. Two hundred and twenty strains isolated by culture from 20 root canals were subjected to DNA extraction and amplification of the 16S rRNA gene (PCR), followed by sequencing. The resulting nucleotide sequences were compared to the GenBank database from the National Center of Biotechnology Information through BLAST. Strains not identified by sequencing were submitted to clonal analysis. The association of microbiological findings with clinical features and the association between microbial species were also investigated. Fifty-nine different cultivable bacteria were identified by 16S rRNA gene sequencing, belonging to 6 phyla, with an average number of 6 species per root canal. Molecular approaches allowed identification of 99% of isolates. The most frequently identified bacteria were Prevotella spp., Pseudoramibacter alactolyticus, Parvimonas micra, Dialister invisus, Filifactor alocis, and Peptostreptococcus stomatis. Positive association was found between Prevotella buccae and Pseudoramibacter alactolyticus and between Parvimonas micra and Prevotella nigrescens (both p<0.05). It was concluded that the microbiota of infected root canals associated with acute apical abscess is diverse and heterogeneous, composed mainly of anaerobic Gram-negative bacteria, with the great majority belonging to the phyla Firmicutes and Bacteroidetes.en
dc.description.abstractO objetivo deste estudo foi investigar a composição bacteriana de canais radiculares associados com abscesso apical agudo através de identificação molecular (16S rRNA) de bactérias cultiváveis. Duzentas e vinte cepas, de 20 casos, isoladas por cultura foram submetidas a extração de DNA e amplificação do gene 16S rRNA (PCR), seguido de sequenciamento. As sequências de nucleotídeos obtidas foram comparadas com o banco de dados (GenBank) do National Center of Biotechnology Information através do BLAST. Cepas não identificadas pelo sequenciamento foram submetidas à clonagem. Associação de achados microbiológicos e características clínicas e associação entre espécies bacterianas também foram investigadas. Cinquenta e nove bactérias cultiváveis diferentes foram identificadas pelo sequenciamento do gene 16S rRNA, pertencentes a 6 filos, numa média de 6 espécies por canal. Método molecular permitiu identificação de 99% das cepas isoladas. As bactérias mais frequentes foram Prevotella spp., Pseudoramibacter alactolyticus, Parvimonas micra, Dialister invisus, Filifactor alocis, Peptostreptococcus stomatis. Associação positiva foi encontrada entre Prevotella buccae e Pseudoramibacter alactolyticus, e entre Parvimonas micra e Prevotella nigrescens (p<0,05). Foi concluído que a microbiota de canais radiculares infectados associados com abscesso apical agudo é diversa e heterogênea, composta principalmente por anaeróbios Gram-negativos, pertencentes aos filos Firmicutes e Bacteroidetes.pt_BR
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian dental journal. Ribeirão Preto. Vol. 27, no. 3 (June 2016), p. 318-324pt_BR
dc.rightsOpen Accessen
dc.subjectEndodontiapt_BR
dc.subjectRoot canalsen
dc.subjectEndodonticsen
dc.subjectBactériaspt_BR
dc.subjectCanais radiculares : Bacteriologiapt_BR
dc.subjectMicroorganismsen
dc.subjectAbscessen
dc.subjectSequencingen
dc.titleMolecular identification of cultivable bacteria from infected root canals associated with acute apical abscesspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000994329pt_BR
dc.type.originNacionalpt_BR


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