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dc.contributor.authorReolon, Luciano Antoniopt_BR
dc.contributor.authorMartello, Carolina Lumertzpt_BR
dc.contributor.authorSchrank, Irene Silveirapt_BR
dc.contributor.authorFerreira, Henrique Bunselmeyerpt_BR
dc.date.accessioned2015-04-15T01:58:23Zpt_BR
dc.date.issued2014pt_BR
dc.identifier.issn1932-6203pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/115357pt_BR
dc.description.abstractThe characterization of the repertoire of proteins exposed on the cell surface by Mycoplasma hyopneumoniae (M. hyopneumoniae), the etiological agent of enzootic pneumonia in pigs, is critical to understand physiological processes associated with bacterial infection capacity, survival and pathogenesis. Previous in silico studies predicted that about a third of the genes in the M. hyopneumoniae genome code for surface proteins, but so far, just a few of them have experimental confirmation of their expression and surface localization. In this work, M. hyopneumoniae surface proteins were labeled in intact cells with biotin, and affinity-captured biotin-labeled proteins were identified by a gel-based liquid chromatographytandem mass spectrometry approach. A total of 20 gel slices were separately analyzed by mass spectrometry, resulting in 165 protein identifications corresponding to 59 different protein species. The identified surface exposed proteins better defined the set of M. hyopneumoniae proteins exposed to the host and added confidence to in silico predictions. Several proteins potentially related to pathogenesis, were identified, including known adhesins and also hypothetical proteins with adhesin-like topologies, consisting of a transmembrane helix and a large tail exposed at the cell surface. The results provided a better picture of the M. hyopneumoniae cell surface that will help in the understanding of processes important for bacterial pathogenesis. Considering the experimental demonstration of surface exposure, adhesion-like topology predictions and absence of orthologs in the closely related, non-pathogenic species Mycoplasma flocculare, several proteins could be proposed as potential targets for the development of drugs, vaccines and/or immunodiagnostic tests for enzootic pneumonia.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofPLoS ONE. San Francisco. Vol. 9, no. 11 (Nov. 2014), e112596, p. 1-7pt_BR
dc.rightsOpen Accessen
dc.subjectMycoplasma hyopneumoniaept_BR
dc.titleSurvey of surface proteins from the pathogenic Mycoplasma hyopneumoniae strain 7448 using a biotin cell surface labeling approachpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000948358pt_BR
dc.type.originEstrangeiropt_BR


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