Mostrar registro simples

dc.contributor.authorParis, Fernanda dept_BR
dc.contributor.authorBeck, Carolinept_BR
dc.contributor.authorNunes, Luciana de Souzapt_BR
dc.contributor.authorMachado, Alice Beatriz Mombach Pinheiropt_BR
dc.contributor.authorPaiva, Rodrigo Minutopt_BR
dc.contributor.authorMenezes, Denise da Silvapt_BR
dc.contributor.authorPires, Márcia Rosanept_BR
dc.contributor.authorSantos, Rodrigo Pires dospt_BR
dc.contributor.authorKuchenbecker, Ricardo de Souzapt_BR
dc.contributor.authorBarth, Afonso Luispt_BR
dc.date.accessioned2015-03-07T01:57:13Zpt_BR
dc.date.issued2014pt_BR
dc.identifier.issn1743-422Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/111838pt_BR
dc.description.abstractBackground: Respiratory syncytial virus (RSV) is the main cause of lower respiratory tract illness in children worldwide. Molecular analyses show two distinct RSV groups (A and B) that comprise different genotypes. This variability contributes to the capacity of RSV to cause yearly outbreaks. These RSV genotypes circulate within the community and within hospital wards. RSV is currently the leading cause of nosocomial respiratory tract infections in pediatric populations. The aim of this study was to evaluate the G protein gene diversity of RSV amplicons. Methods: Nasopharyngeal aspirate samples were collected from children with nosocomial or community-acquired infections. Sixty-three RSV samples (21 nosocomial and 42 community-acquired) were evaluated and classified as RSV-A or RSV-B by real-time PCR. Sequencing of the second variable region of the G protein gene was performed to establish RSV phylogenetics. Results: We observed co-circulation of RSV-A and RSV-B, with RSV-A as the predominant group. All nosocomial and community-acquired RSV-A samples were from the same phylogenetic group, comprising the NA1 genotype, and all RSV-B samples (nosocomial and community-acquired) were of the BA4 genotype. Therefore, in both RSV groups (nosocomial and community-acquired), the isolates belonged to only one genotype in circulation. Conclusions: This is the first study to describe circulation of the NA1 RSV genotype in Brazil. Furthermore, this study showed that the BA4 genotype remains in circulation. Deciphering worldwide RSV genetic variability will aid vaccine design and development.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofVirology journal. London. Vol. 11 (Feb. 2014), 36, 6 p.pt_BR
dc.rightsOpen Accessen
dc.subjectRespiratory syncytial virusen
dc.subjectVírus sinciciais respiratóriospt_BR
dc.subjectInfecção hospitalarpt_BR
dc.subjectNosocomial infectionen
dc.subjectG-proteinen
dc.subjectProteínas de ligação ao GTPpt_BR
dc.subjectEpidemiologia molecularpt_BR
dc.subjectGenetic variabilityen
dc.subjectFarmáciapt_BR
dc.subjectMolecular epidemiologyen
dc.titleEvaluation of respiratory syncytial virus group A and B genotypes among nosocomial and community-acquired pediatric infections in southern Brazilpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000953329pt_BR
dc.type.originEstrangeiropt_BR


Thumbnail
   

Este item está licenciado na Creative Commons License

Mostrar registro simples