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dc.contributor.authorYurgel, Virgina Campellopt_BR
dc.contributor.authorOliveira, Catiúscia Padilha dept_BR
dc.contributor.authorBegnini, Karine Rechpt_BR
dc.contributor.authorSchultze, Eduardapt_BR
dc.contributor.authorThurow, Helena Strelowpt_BR
dc.contributor.authorLeon, Priscila Marques Moura dept_BR
dc.contributor.authorDellagostin, Odir Antôniopt_BR
dc.contributor.authorCampos, Vinicius Fariaspt_BR
dc.contributor.authorBeck, Ruy Carlos Ruverpt_BR
dc.contributor.authorGuterres, Silvia Stanisçuaskipt_BR
dc.contributor.authorCollares, Tiago Veiraspt_BR
dc.contributor.authorPohlmann, Adriana Raffinpt_BR
dc.contributor.authorSeixas, Fabiana Kömmlingpt_BR
dc.date.accessioned2014-06-28T02:09:04Zpt_BR
dc.date.issued2014pt_BR
dc.identifier.issn1178-2013pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/97020pt_BR
dc.description.abstractBreast cancer is the most frequent cancer affecting women. Methotrexate (MTX) is an antimetabolic drug that remains important in the treatment of breast cancer. Its efficacy is compromised by resistance in cancer cells that occurs through a variety of mechanisms. This study evaluated apoptotic cell death and cell cycle arrest induced by an MTX derivative (MTX diethyl ester [MTX(OEt)2]) and MTX(OEt)2-loaded lipid-core nanocapsules in two MTX-resistant breast adenocarcinoma cell lines, MCF-7 and MDA-MB-231. The formulations prepared presented adequate granulometric profile. The treatment responses were evaluated through flow cytometry. Relying on the mechanism of resistance, we observed different responses between cell lines. For MCF-7 cells, MTX(OEt)2 solution and MTX(OEt)2-loaded lipid-core nanocapsules presented significantly higher apoptotic rates than untreated cells and cells incubated with unloaded lipid-core nanocapsules. For MDA-MB-231 cells, MTX(OEt)2-loaded lipid-core nanocapsules were significantly more efficient in inducing apoptosis than the solution of the free drug. S-phase cell cycle arrest was induced only by MTX(OEt)2 solution. The drug nanoencapsulation improved apoptosis induction for the cell line that presents MTX resistance by lack of transport receptors.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofInternational Journal of Nanomedicine. Macclesfield, UK. Vol. 9, no. 1 (2014), p. 1583-1591pt_BR
dc.rightsOpen Accessen
dc.subjectMTX derivativeen
dc.subjectMetotrexatopt_BR
dc.subjectResistanceen
dc.subjectNeoplasias da mamapt_BR
dc.subjectApoptotic cell deathen
dc.subjectCell cycle arresten
dc.subjectNanocarriersen
dc.subjectDrug deliveryen
dc.subjectDrug targetingen
dc.titleMethotrexate diethyl ester-loaded lipid-core nanocapsules in aqueous solution increased antineoplastic effects in resistant breast cancer cell linept_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000916726pt_BR
dc.type.originEstrangeiropt_BR


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