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dc.contributor.authorBueno, Verônica La Cruzpt_BR
dc.contributor.authorBastos, Henrique Boll de Araujopt_BR
dc.contributor.authorCenteno, Luiz Augusto Machadopt_BR
dc.contributor.authorKretzmann Filho, Nelson Alexandrept_BR
dc.contributor.authorMattos, Rodrigo Costapt_BR
dc.contributor.authorRechsteiner, Sandra Mara da Encarnação Fialapt_BR
dc.date.accessioned2024-11-26T06:57:00Zpt_BR
dc.date.issued2024pt_BR
dc.identifier.issn1806-9614pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/281495pt_BR
dc.description.abstractThis study aims to investigate the gene expression of sperm-borne phospholipase C zeta (PLCζ), WW domain-binding protein 2N-Terminal Like (WBP2NL), and Tumor necrosis factor (TNF-α), as a negative control, in spermatozoa and their relationship with fertility and seminal quality in stallions. Ejaculates from 40 Criollo stallions were used, whose fertility was assessed on the basis of their pregnancy rate per cycle in at least two breeding seasons. Pregnancy rates ranged from 20% to 90% and were used to divide the stallions into two groups: High rates (≥ 50%) (n = 25), and Low rates (< 50%) (n = 15). A computer-assisted sperm analysis system - (CASA) analyzed semen after collection. Also were evaluated the physical and functional integrity of the plasmatic membrane and sperm morphology alterations. All stallions expressed PLCζ, WBP2NL, and TNF-α. PLCζ positively correlates with conception rate, total motility (TM), progressive motility (PM), plasmatic membrane functionality, and integrity. A simple linear regression was detected between pregnancy rate and PLCζ expression (P = 0.003), TM (P < 0.001) and PM (P < 0.001). PLCζ gene expression was higher (P = 0,012) in the High rates group than in the Low group. WBP2NL and TNF-α did not correlate with seminal quality and stallion’s fertility. It was concluded that PLCζ gene expression in the spermatozoa might be used as a biomarker of fertility and seminal quality in stallions. Parameters of sperm kinetics also showed, positive correlation between TM, PM and pregnancy rate.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofAnimal reproduction. Belo Horizonte. Vol. 21, no. 1 (2024), e20230088, 10 p.pt_BR
dc.rightsOpen Accessen
dc.subjectGene expressionen
dc.subjectExpressão gênicapt_BR
dc.subjectFosfolipases tipo Cpt_BR
dc.subjectConception rateen
dc.subjectSeminal qualityen
dc.subjectProteínas do espermatozoidept_BR
dc.subjectStallionsen
dc.subjectTaxa de prenhezpt_BR
dc.subjectMotilidade espermáticapt_BR
dc.subjectMorfologia espermáticapt_BR
dc.subjectEquinospt_BR
dc.subjectBiomarcadorespt_BR
dc.titlePLCζ, WBP2NL and TNF-α expression in spermatozoa is associated with stallion fertility and seminal quality?pt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001210148pt_BR
dc.type.originNacionalpt_BR


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