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dc.contributor.authorVieira, Tatiana Reginapt_BR
dc.contributor.authorCavinatto, Esther Ferrazzapt_BR
dc.contributor.authorCibulski, Samuel Paulopt_BR
dc.contributor.authorSilva, Núbia Michelle Vieira dapt_BR
dc.contributor.authorBorba, Mauro Riegertpt_BR
dc.contributor.authorOliveira, Celso José Bruno dept_BR
dc.contributor.authorCardoso, Marisa Ribeiro de Itapemapt_BR
dc.date.accessioned2024-01-13T03:41:44Zpt_BR
dc.date.issued2023pt_BR
dc.identifier.issn0032-5791pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/270963pt_BR
dc.description.abstractThe aim of this study was to investigate the microbial composition, and the profiles of antimicro-bial resistance genes (ARGs, resistome) and mobile genetic elements (mobilome) of retail chicken carcasses originated from conventional intensive production systems (CO), certified antimicrobial-free intensive production systems (AF), and certified organic production systems with restricted antimicrobial use (OR). DNA samples were collected from 72 chicken carcasses according to a cross-sectional study design. Shot-gun metagenomics was performed by means of Illumina high throughput DNA sequencing followed by downstream bioinformatic analyses. Gammaproteobacteria was the most abundant bacterial class in all groups. Although CO, AF, and OR did not differ in terms of alpha-and beta-microbial diversity, the abundance of some taxa differed significantly across the groups, including spoilage-associated organisms such as Pseudomonas and Acinetobacter. The co-resistome comprised 29 ARGs shared by CO, AF and OR, including genes conferring resistance to beta-lactams (blaACT-8, 10, 13, 29; blaOXA-212; blaOXA-275 and ompA), aminoglycosides (aph(30)-IIIa, VI, VIa and spd), tetracyclines (tet KL (W/N/W and M), lincosamides (inu A,C) and fosfomycin (fosA). ARGs were significantly less abundant (P < 0.05) in chicken carcasses from AF and OR compared with CO. Regarding mobile genetic elements (MGEs), transposases accounted for 97.2% of the mapped genes. A higher abundance (P = 0.037) of MGEs was found in CO compared to OR. There were no significant differences in ARGs or MGEs diversity among groups according to the Simpson = s index. In summary, retail frozen chicken carcasses from AF and OR systems show similar ARGs, MGEs and microbiota profiles compared with CO, even though the abundance of ARGs and MGEs was higher in chicken carcasses from CO, probably due to a higher selective pressure.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofPoultry science. Amsterdam. Vol. 102, no. 11 (Nov. 2023), 103002, 11 p.pt_BR
dc.rightsOpen Accessen
dc.subjectAntimicrobial resistanceen
dc.subjectMicrobiotapt_BR
dc.subjectPoultry industryen
dc.subjectResistência a antimicrobianospt_BR
dc.subjectFood safetyen
dc.subjectElementos genéticos móveispt_BR
dc.subjectCriação intensivapt_BR
dc.subjectOne healthen
dc.subjectProdução orgânicapt_BR
dc.subjectMetagenomicsen
dc.subjectCarcaça de frangopt_BR
dc.subjectMetagenômicapt_BR
dc.titleComparative resistome, mobilome, andmicrobial composition of retail chicken originated from conventional, organic, and antibiotic-free production systemspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001193862pt_BR
dc.type.originEstrangeiropt_BR


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