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dc.contributor.authorZago, Fabiano Carminattipt_BR
dc.contributor.authorSchutz, Luis Fernandopt_BR
dc.contributor.authorGerger, Renato Pereira da Costapt_BR
dc.contributor.authorAguiar, Luis Henrique dept_BR
dc.contributor.authorPinzón Osorio, César Augustopt_BR
dc.contributor.authorMezzalira, Alceupt_BR
dc.contributor.authorRodrigues, José Luiz Rigopt_BR
dc.contributor.authorForell, Fabianapt_BR
dc.contributor.authorBertolini, Marcelopt_BR
dc.date.accessioned2024-01-12T03:29:25Zpt_BR
dc.date.issued2023pt_BR
dc.identifier.issn1806-9614pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/270915pt_BR
dc.description.abstractThe aim of this study was to compare embryo production efficiency in Flemish and Holstein donor females using ovum pick-up and in vitro fertilization (OPU-IVF) or in vivo production (superovulation; SOV) procedures. The study was conducted using a split-plot design, with eight Flemish and eight Holstein non-lactating cycling females. Females were subjected to ten weekly OPU/IVF sessions and/or two SOV/embryo collections sessions at a 63-day interval, for a total of 160 OPU-IVF and 32 SOV sessions. Mean numbers of follicles and corpora lutea, and cumulus-oocyte complex (COC) recovery rates were similar between breeds after the OPU and SOV sessions. However, Flemish donors yielded better quality grade II COCs (301, 41.9%) than Holstein females (609, and 202, 33.1%). Also, cleavage and blastocyst rates, and the total number and the mean number of viable embryos obtained after OPU-IVF were higher in Flemish (49.6% and 11.8%, and 63 and 11.8 per donor, respectively) than in Holstein (32.8% and 7.2%, and 34 and 7.2 per donor, respectively) females. Flemish females were also more efficient in yielding viable embryos after SOV (111, 7.3 per donor) than Holstein (48, 3.3 per donor) females. Overall, Flemish donor females had better responses to OPU-IVF or SOV procedures than Holstein counterparts. Irrespective of the breeds, SOV procedures were more efficient than OPU-IVF in yielding more viable embryos, under the conditions of this study. Both reproductive procedures were useful tools for the genetic conservation of the Flemish cattle breed in Southern Brazil.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofAnimal reproduction. Belo Horizonte. Vol. 20, no. 3 (2023), e20230080, 15 p.pt_BR
dc.rightsOpen Accessen
dc.subjectDesempenho reprodutivopt_BR
dc.subjectIn vitro fertilizationen
dc.subjectOvum pick-upen
dc.subjectFertilização in vitropt_BR
dc.subjectMultiple ovulationen
dc.subjectSuperovulaçãopt_BR
dc.subjectBreed conservationen
dc.subjectEmbrioespt_BR
dc.subjectRaças de bovinospt_BR
dc.subjectCattleen
dc.titleIn vitro and in vivo embryo production efficiency in Flemish and Holstein donor femalespt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001193295pt_BR
dc.type.originNacionalpt_BR


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