Interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape : implications for detection
dc.contributor.author | Serra, Pedro M. | pt_BR |
dc.contributor.author | Bertolini, Edson | pt_BR |
dc.contributor.author | Martínez, M. Carmen | pt_BR |
dc.contributor.author | Cambra, Mariano | pt_BR |
dc.contributor.author | Pedauyé, Ricardo Flores | pt_BR |
dc.date.accessioned | 2023-08-16T03:33:51Z | pt_BR |
dc.date.issued | 2017 | pt_BR |
dc.identifier.issn | 2045-2322 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/263661 | pt_BR |
dc.description.abstract | Natural populations of peach latent mosaic viroid (PLMVd) are complex mixtures of variants. During routine testing, TaqMan rtRT-PCR and RNA gel-blot hybridization produced discordant results with some PLMVd isolates. Analysis of the corresponding populations showed that they were exclusively composed of variants (of class II) with a structural domain different from that of the reference and many other variants (of class I) targeted by the TaqMan rtRT-PCR probe. Bioassays in peach revealed that a representative PLMVd variant of class II replicated without symptoms, generated a progeny with low nucleotide diversity, and, intriguingly, outcompeted a representative symptomatic variant of class I when co-inoculated in equimolecular amounts. A number of informative positions associated with the higher fitness of variants of class II have been identified, and novel sets of primers and probes for universal or specific TaqMan rtRT-PCR detection of PLMVd variants have been designed and tested. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Scientific reports. London. vol. 7 (Feb. 2017), [art.] 42825, 10 p. | pt_BR |
dc.rights | Open Access | en |
dc.subject | Doença de planta | pt_BR |
dc.subject | Vírus | pt_BR |
dc.subject | Pêssego | pt_BR |
dc.title | Interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape : implications for detection | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 001172764 | pt_BR |
dc.type.origin | Estrangeiro | pt_BR |
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