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dc.contributor.authorTirloni, Lucaspt_BR
dc.contributor.authorSabadin, Gabriela Alvespt_BR
dc.contributor.authorVaz Junior, Itabajara da Silvapt_BR
dc.contributor.authorRibeiro, José M. C.pt_BR
dc.date.accessioned2020-12-11T04:11:18Zpt_BR
dc.date.issued2020pt_BR
dc.identifier.issn2045-2322pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/216344pt_BR
dc.description.abstractTo further obtain insights into the Rhipicephalus microplus transcriptome, we used RNA-seq to carry out a study of expression in (i) embryos; (ii) ovaries from partially and fully engorged females; (iii) salivary glands from partially engorged females; (iv) fat body from partially and fully engorged females; and (v) digestive cells from partially, and (vi) fully engorged females. We obtained > 500 million Illumina reads which were assembled de novo, producing > 190,000 contigs, identifying 18,857 coding sequences (CDS). Reads from each library were mapped back into the assembled transcriptome giving a view of gene expression in different tissues. Transcriptomic expression and pathway analysis showed that several genes related in blood digestion and host-parasite interaction were overexpressed in digestive cells compared with other tissues. Furthermore, essential genes for the cell development and embryogenesis were overexpressed in ovaries. Taken altogether, these data offer novel insights into the physiology of production and role of saliva, blood digestion, energy metabolism, and development with submission of 10,932 novel tissue/cell specific CDS to the NCBI database for this important tick species.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofScientific reports. London. Vol. 10 (2020), 18296, 17 p.pt_BR
dc.rightsOpen Accessen
dc.subjectRhipicephalus micropluspt_BR
dc.subjectTranscriptomapt_BR
dc.subjectExpressão gênicapt_BR
dc.subjectTecido animalpt_BR
dc.subjectFisiologia animalpt_BR
dc.subjectRNAseqpt_BR
dc.titleA physiologic overview of the organ‑specific transcriptome of the cattle tick Rhipicephalus micropluspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001119390pt_BR
dc.type.originEstrangeiropt_BR


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