Avaliação de heterorresistência ao imipenem em enterobactérias produtoras e não-produtoras de kpc
dc.contributor.advisor | Barth, Afonso Luis | pt_BR |
dc.contributor.author | Nodari, Carolina Silva | pt_BR |
dc.date.accessioned | 2016-07-28T02:16:06Z | pt_BR |
dc.date.issued | 2013 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/144032 | pt_BR |
dc.description.abstract | The misdetection of Klebsiella pneumoniae carbapenemase (KPC) may lead to treatment failure and favour the spread of this resistance mechanism. Heteroresistance is an antimicrobial resistance in a subset of an isolate considered to be susceptible by conventional testing. The aim of this study was to evaluate the presence of heteroresistance to imipenem in KPC-producing and non-producing K. pneumoniae and E. coli. After the determination of the minimum inhibitory concentration (MIC) to imipenem by broth microdilution, each isolate with MIC lower than 4 mg L-1 was subcultured in plates containing imipenem ranging from 0.25 mg L-1 to 32 mg L-1. Isolates with growth in imipenem concentrations two fold dilutions above the original MIC were considered heteroresistant. The KPC group consisted of 10 isolates, while the non-KPC group consisted of four isolates and distinct results were observed among them: all KPC-producing isolates presented heteroresistance to imipenem, while for the KPC-non-producing isolates this phenomenon was not observed. Interestingly, we also found heteroresistance in E. coli isolates, which, to best of our knowledge, was not reported as yet. Our results indicated a possible relationship between heteroresistance and KPC production. The clinical implications of appearing of heteroresistant subpopulations remains to be investigated, considering that this phenomenon seems to be frequent in Gram-negative rods, including members, such as E. coli, in which this phenomenon is still rarely investigated. | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | por | pt_BR |
dc.rights | Open Access | en |
dc.subject | Imipenem | pt_BR |
dc.subject | KPC | en |
dc.subject | E. coli | en |
dc.subject | Resistência microbiana a medicamentos | pt_BR |
dc.subject | Imipenem | en |
dc.subject | Klebsiella pneumoniae | pt_BR |
dc.subject | Heteroresistance | en |
dc.title | Avaliação de heterorresistência ao imipenem em enterobactérias produtoras e não-produtoras de kpc | pt_BR |
dc.title.alternative | Imipenem heteroresistance: evaluation of Klebsiella pneumoniae carbapenemase-producing and non-producing K. pneumoniae and E. coli | en |
dc.type | Trabalho de conclusão de graduação | pt_BR |
dc.contributor.advisor-co | Ribeiro, Vanessa Bley | pt_BR |
dc.identifier.nrb | 000914725 | pt_BR |
dc.degree.grantor | Universidade Federal do Rio Grande do Sul | pt_BR |
dc.degree.department | Faculdade de Farmácia | pt_BR |
dc.degree.local | Porto Alegre, BR-RS | pt_BR |
dc.degree.date | 2013 | pt_BR |
dc.degree.graduation | Farmácia | pt_BR |
dc.degree.level | graduação | pt_BR |
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