Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
dc.contributor.author | Rech, Rosane | pt_BR |
dc.contributor.author | Ayub, Marco Antônio Záchia | pt_BR |
dc.date.accessioned | 2014-11-29T02:20:52Z | pt_BR |
dc.date.issued | 2006 | pt_BR |
dc.identifier.issn | 0104-6632 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/107573 | pt_BR |
dc.description.abstract | Saccharomyces cerevisiae strain W303 was transformed with two yeast integrative plasmids containing Kluyveromyces lactis LAC4 and LAC12 genes that codify β-galactosidase and lactose permease respectively. The BLR030 recombinant strain was selected due to its growth and β-galactosidase production capacity. Different culture media based on deproteinized cheese whey (DCW) were tested and the best composition (containing DCW, supplemented with yeast extract 1 %, and peptone 3 % (w/v)) was chosen for bioreactor experiments. Batch, and fed-batch cultures with linear ascending feeding for 25 (FB25), 35 (FB35), and 50 (FB50) hours, were performed. FB35 and FB50 produced the highest β-galactosidase specific activities (around 1,800 U/g cells), and also the best productivities (180 U/L.h). Results show the potential use of fed-batch cultures of recombinant S. cerevisiae on industrial applications using supplemented whey as substrate. | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | por | pt_BR |
dc.relation.ispartof | Brazilian Journal of Chemical Engineering. São Paulo. vol. 23, n. 4 (oct./dec. 2006), p. 435-442 | pt_BR |
dc.rights | Open Access | en |
dc.subject | Recombinant Saccharomyces cerevisiae | en |
dc.subject | Saccharomyces cerevisiae recombinante | pt_BR |
dc.subject | Soro de leite | pt_BR |
dc.subject | β-galactosidase | en |
dc.subject | Cheese-whey | en |
dc.subject | beta-Galactosidase | pt_BR |
dc.subject | Fed-batch cultivation | en |
dc.title | Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 000567377 | pt_BR |
dc.type.origin | Nacional | pt_BR |
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