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dc.contributor.authorFerreira, Fernanda Stodutopt_BR
dc.contributor.authorHorvath, Mariana Bandeirapt_BR
dc.contributor.authorTondo, Eduardo Cesarpt_BR
dc.date.accessioned2014-08-12T02:10:47Zpt_BR
dc.date.issued2013pt_BR
dc.identifier.issn1517-8382pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/100141pt_BR
dc.description.abstractThe objective of the present study was to assess the growth and the recovery of Salmonella (S.) Enteritidis SE86 in different diluents, culture media and using different plating methods after the exposure to 200 mg/kg sodium dichloroisocyanurate (NaDCC). Before and after NaDCC exposure, SE86 was cultured at 30 °C and 7 °C in the following diluents: Peptone water (P), Saline solution (SaS), Peptone water+Saline solution (P+SaS), Peptone water+Tween 80+Lecithin+Sodium thiosulfate (P+N) and Saline solution+Tween 80+Lecithin+Sodium thiosulfate (SaS+N). The SaS diluent was chosen because it was able to maintain cells viable without growth and was further used for plating SE86 on non selective medium (Tryptic Soy Agar-TSA) and on selective media (Mannitol Lysine Crystal Violet Brilliant Green Agar-MLCB; Brilliant Green Agar-BGA; Salmonella Shigella Agar-SS and Xylose Lysine Dextrose–XLD). The Thin Agar Layer method (TAL) i.e., selective media overlayed with non selective TSA was also evaluated. Results indicated that SE86 not exposed to NaDCC was able to grow in P, P+N, SaS+N and P+SaS, but not in SaS, that was able to maintain cells viable. SE86 exposed to NaDCC demonstrated similar counts after dilution in SaS and the plating on non selective TSA, selective media MLCB, BGA, SS and XLD and on TAL media. SE86, S. Typhimurium and S. Bredeney, exposed or not exposed to NaDCC, showed no significant differences in counts on TSA, XLD and XLD overlayed with TSA, suggesting that all those media may be used to quantify NaDCC-exposed Salmonella by plating method.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of microbiology. São Paulo. Vol. 44, no. 3 (July/Sept. 2013), p. 785-790pt_BR
dc.rightsOpen Accessen
dc.subjectSalmonella enteritidis SE86en
dc.subjectSalmonella enteritidispt_BR
dc.subjectSalmonella entericapt_BR
dc.subjectDiluentsen
dc.subjectPlating methodsen
dc.subjectStressed cellsen
dc.subjectSodium dichloroisocyanurateen
dc.titleAssessing the growth and recovery of Salmonella Enteritidis SE86 after sodium dichloroisocyanurate exposurept_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000921482pt_BR
dc.type.originNacionalpt_BR


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