Genotoxicity of Nicotiana tabacum leaves on Helix aspersa
dc.contributor.author | Silva, Fernanda Rabaioli da | pt_BR |
dc.contributor.author | Erdtmann, Bernardo | pt_BR |
dc.contributor.author | Dalpiaz, Tiago | pt_BR |
dc.contributor.author | Nunes, Emilene | pt_BR |
dc.contributor.author | Ferraz, Alexandre de Barros Falcão | pt_BR |
dc.contributor.author | Martins, Tales Leandro Costa | pt_BR |
dc.contributor.author | Dias, Johnny Ferraz | pt_BR |
dc.contributor.author | Rosa, Darlan Pase da | pt_BR |
dc.contributor.author | Porawski, Marilene | pt_BR |
dc.contributor.author | Bona, Silvia Regina | pt_BR |
dc.contributor.author | Silva, Juliana da | pt_BR |
dc.date.accessioned | 2014-02-28T01:50:52Z | pt_BR |
dc.date.issued | 2013 | pt_BR |
dc.identifier.issn | 1415-4757 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/87983 | pt_BR |
dc.description.abstract | Tobacco farmers are routinely exposed to complex mixtures of inorganic and organic chemicals present in tobacco leaves. In this study, we examined the genotoxicity of tobacco leaves in the snail Helix aspersa as a measure of the risk to human health. DNA damage was evaluated using the micronucleus test and the Comet assay and the concentration of cytochrome P450 enzymes was estimated. Two groups of snails were studied: one fed on tobacco leaves and one fed on lettuce (Lactuca sativa L) leaves (control group). All of the snails received leaves (tobacco and lettuce leaves were the only food provided) and water ad libitum. Hemolymph cells were collected after 0, 24, 48 and 72 h. The Comet assay and micronucleus test showed that exposure to tobacco leaves for different periods of time caused significant DNA damage. Inhibition of cytochrome P450 enzymes occurred only in the tobacco group. Chemical analysis indicated the presence of the alkaloid nicotine, coumarins, saponins, flavonoids and various metals. These results show that tobacco leaves are genotoxic in H. aspersa and inhibit cytochrome P450 activity, probably through the action of the complex chemical mixture present in the plant. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Genetics and molecular biology. Vol. 36, n. 2 (Jun. 2013), p. 269-275 | pt_BR |
dc.rights | Open Access | en |
dc.subject | Genotoxicidade | pt_BR |
dc.subject | comet assay | en |
dc.subject | genotoxicity | en |
dc.subject | Helix aspersa | pt_BR |
dc.subject | micronucleus test | en |
dc.subject | Nicotiana tabacum | pt_BR |
dc.subject | Helix aspersa | en |
dc.subject | Tabaco | pt_BR |
dc.subject | Nicotiana tabacum | en |
dc.subject | tobacco leaves | en |
dc.title | Genotoxicity of Nicotiana tabacum leaves on Helix aspersa | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 000896878 | pt_BR |
dc.type.origin | Nacional | pt_BR |
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