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dc.contributor.authorCoutinho, Vivian de Lima Spodept_BR
dc.contributor.authorPaiva, Rodrigo Minutopt_BR
dc.contributor.authorReiter, Keli Cristinept_BR
dc.contributor.authorParis, Fernanda dept_BR
dc.contributor.authorBarth, Afonso Luispt_BR
dc.contributor.authorMachado, Alice Beatriz Mombach Pinheiropt_BR
dc.date.accessioned2014-02-28T01:50:33Zpt_BR
dc.date.issued2010pt_BR
dc.identifier.issn1413-8670pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/87955pt_BR
dc.description.abstractIntroduction: Resistance to macrolides, lincosamides and streptogramins B (MLSB antibiotics) in staphylococci may be due to modification in ribosomal target methylase encoded by erm genes. The expression of MLSB resistance lead to three phenotypes, namely constitutive resistance (cMLSB), inducible resistance (iMLSB), and resistance only to macrolides and streptogramins B (MSB). The iMLSB resistance is the most difficult to detect in the clinical laboratory. Objective: This study investigated the expression of MLSB resistance and the prevalence of the erm genes among 152 clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus (CNS) from Hospital de Clínicas de Porto Alegre. Methods: Primary MLSB resistance was detected by the disk diffusion method. Isolates with iMLSB phenotype were tested by double-disk induction method. All isolates were tested by a genotypic assay, PCR with specific primers. Results: A total of 46.7% of staphylococci were positive for cMLSB; 3.3% for iMLSB and 3.3% for MSB. One or more erm genes were present in 50.1% of isolates. The gene ermA was detected in 49 isolates, ermC in 29 and ermB in 3. Conclusion: The prevalence of the ermA, ermB and ermC genes were 29.6%, 17.1% and 0.66% respectively, and constitutive resistance was the most frequent as compared to the other two phenotypes.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofThe Brazilian journal of infectious diseases. Vol. 14, no. 6 (2010), p. 564-568pt_BR
dc.rightsOpen Accessen
dc.subjectMacrolídeospt_BR
dc.subjecterm genesen
dc.subjectmacrolidesen
dc.subjectStaphylococcuspt_BR
dc.subjectEritromicinapt_BR
dc.subjectresistanceen
dc.subjectStaphylococcusen
dc.titleDistribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolatespt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000872523pt_BR
dc.type.originNacionalpt_BR


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