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dc.contributor.authorCechetti, Fernandapt_BR
dc.contributor.authorWorm, Paulo Valdecipt_BR
dc.contributor.authorSilva, Lenir Orlandi Pereirapt_BR
dc.contributor.authorSiqueira, Ionara Rodriguespt_BR
dc.contributor.authorNetto, Carlos Alexandrept_BR
dc.date.accessioned2011-08-04T06:01:26Zpt_BR
dc.date.issued2010pt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/30588pt_BR
dc.description.abstractPermanent bilateral occlusion of the common carotid arteries (2VO) in the rat has been established as a valid experimental model to investigate the effects of chronic cerebral hypoperfusion on cognitive function and neurodegenerative processes. Our aim was to compare the cognitive and morphological outcomes following the standard 2VO procedure, in which there is concomitant artery ligation, with those of a modified protocol, with a 1-week interval between artery occlusions to avoid an abrupt reduction of cerebral blood flow, as assessed by animal performance in the water maze and damage extension to the hippocampus and striatum. Male Wistar rats (N = 47) aged 3 months were subjected to chronic hypoperfusion by permanent bilateral ligation of the common carotid arteries using either the standard or the modified protocol, with the right carotid being the first to be occluded. Three months after the surgical procedure, rat performance in the water maze was assessed to investigate long-term effects on spatial learning and memory and their brains were processed in order to estimate hippocampal volume and striatal area. Both groups of hypoperfused rats showed deficits in reference (F(8,172) = 7.0951, P < 0.00001) and working spatial memory [2nd (F(2,44) = 7.6884, P < 0.001), 3rd (F(2,44) = 21.481, P < 0.00001) and 4th trials (F(2,44) = 28.620, P < 0.0001)]; however, no evidence of tissue atrophy was found in the brain structures studied. Despite similar behavioral and morphological outcomes, the rats submitted to the modified protocol showed a significant increase in survival rate, during the 3 months of the experiment (P < 0.02).en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 43, no. 12 (Dez. 2010), p. 1178-1183pt_BR
dc.rightsOpen Accessen
dc.subjectChronic cerebral hypoperfusionen
dc.subjectMemóriapt_BR
dc.subjectSpatial memoryen
dc.subjectHipocampopt_BR
dc.subjectHipoperfusão crônicapt_BR
dc.subjectHippocampusen
dc.subjectStriatumen
dc.subjectWater mazeen
dc.titleThe modified 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival ratept_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000773158pt_BR
dc.type.originNacionalpt_BR


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