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dc.contributor.authorSouza, M. S.pt_BR
dc.contributor.authorMachado, Ubiratan Fabrespt_BR
dc.contributor.authorOkamoto, Maristela Mitikopt_BR
dc.contributor.authorBertoluci, Marcello Casacciapt_BR
dc.contributor.authorPonpermeyer, C.pt_BR
dc.contributor.authorLeguisamo, Natalia Mottapt_BR
dc.contributor.authorAzambuja, Felipept_BR
dc.contributor.authorIrigoyen, Maria Claudia Costapt_BR
dc.contributor.authorSchaan, Beatriz D'Agordpt_BR
dc.date.accessioned2010-04-24T04:15:52Zpt_BR
dc.date.issued2008pt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/21227pt_BR
dc.description.abstractDiabetes in spontaneously hypertensive rats is associated with cortical renal GLUT1 and GLUT2 overexpression. Our objective was to evaluate the effect of the angiotensin-converting enzyme blockade on cortical renal GLUT1 and GLUT2 expression, urinary albumin and urinary TGF-β1. Streptozotocin, 50 mg/kg, or citrate buffer (N = 16) was administered as a single injection into the tail vein in adult spontaneously hypertensive rats (~260 g). Thirty days later, these diabetic spontaneously hypertensive rats received ramipril by gavage: 0.01 mg·kg-1·day-1 (D0.01, N = 14), 1 mg·kg-1·day-1 (D1, N = 9) or water (D, N = 11) for 15 days. Albumin and TGF-β1 (24-h urine), direct arterial pressure, renal tissue angiotensin-converting enzyme activity (fluorometric assay), and GLUT1 and GLUT2 protein levels (Western blot, renal cortex) were determined. Glycemia and glycosuria were higher (P < 0.05) in the diabetic rats compared with controls, but similar between the diabetic groups. Diabetes in spontaneously hypertensive rats lowered renal tissue angiotensin-converting enzyme activity (40%), which was reduced further when higher ramipril doses were used. Diabetes associated with hypertension raised GLUT1 by 28% (P < 0.0001) and GLUT2 by 76% (P = 0.01), and both doses of ramipril equally reduced cortical GLUT1 (D vs D1 and vs D0.01, P ≤ 0.001). GLUT2 levels were reduced in D0.01 (P < 0.05 vs D). Diabetes increased urinary albumin and TGF-β1 urinary excretion, but the 15-day ramipril treatment (with either dose) did not reduce them. In conclusion, ramipril is effective in lowering renal tissue angiotensin-converting enzyme activity, as well as blocking cortical GLUT1 overexpression, which may be beneficial in arresting the development of diabetic nephropathy.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. RiBeirão Preto, SP. Vol. 41, n. 11 (Nov. 2008), p. 960-968pt_BR
dc.rightsOpen Accessen
dc.subjectAngiotensin-converting enzyme inhibitionen
dc.subjectInibidores da enzima conversora da angiotensinapt_BR
dc.subjectDiabetic nephropathyen
dc.subjectDiabetes mellituspt_BR
dc.subjectHipertensãopt_BR
dc.subjectGlucose transporter proteinsen
dc.subjectHypertensionen
dc.subjectStreptozotocinen
dc.titleReduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive ratspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000673195pt_BR
dc.type.originNacionalpt_BR


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