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dc.contributor.authorDarini, A.L.C.pt_BR
dc.contributor.authorMagalhães, V.D.pt_BR
dc.contributor.authorLevy, C.L.pt_BR
dc.contributor.authorBarth, Afonso Luispt_BR
dc.contributor.authorCoscina, A.L.pt_BR
dc.date.accessioned2010-04-24T04:15:32Zpt_BR
dc.date.issued1999pt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/21158pt_BR
dc.description.abstractIn order to evaluate the resolving power of several typing methods to identify relatedness among Brazilian strains of Enterobacter cloacae, we selected twenty isolates from different patients on three wards of a University Hospital (Orthopedics, Nephrology, and Hematology). Traditional phenotyping methods applied to isolates included biotyping, antibiotic sensitivity, phage-typing, and O-serotyping. Plasmid profile analysis, ribotyping, and macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) were used as genotyping methods. Sero- and phage-typing were not useful since the majority of isolates could not be subtyped by these methods. Biotyping, antibiogram and plasmid profile permitted us to classify the samples into different groups depending on the method used, and consequently were not reliable. Ribotyping and PFGE were significantly correlated with the clinical epidemiological analysis. PFGE did not type strains containing nonspecific DNase. Ribotyping was the most discriminative method for typing Brazilian isolates of E. cloacae.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 32, no. 9 (Sept. 1999), p. 1077-1081pt_BR
dc.rightsOpen Accessen
dc.subjectEnterobacter cloacaeen
dc.subjectEnterobacteriaspt_BR
dc.subjectPhage-typingen
dc.subjectEnterobacter cloacaept_BR
dc.subjectSerotypingen
dc.subjectRibotypingen
dc.subjectPulsed-field gel electrophoresisen
dc.subjectNosocomial infectioen
dc.titlePhenotyping and genotyping methods applied to investigate the relatedness of brazilian isolates of enterobacter cloacaept_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000296727pt_BR
dc.type.originNacionalpt_BR


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