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dc.contributor.authorRamos, Grasieli de Oliveirapt_BR
dc.contributor.authorBernardi, Lisianept_BR
dc.contributor.authorLauxen, Isabel da Silvapt_BR
dc.contributor.authorSant'Ana Filho, Manoelpt_BR
dc.contributor.authorHorwitz, Alan Rickpt_BR
dc.contributor.authorLamers, Marcelo Lazzaronpt_BR
dc.date.accessioned2019-09-07T02:33:53Zpt_BR
dc.date.issued2016pt_BR
dc.identifier.issn1932-6203pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/198989pt_BR
dc.description.abstractCell migration is regulated by adhesion to the extracellular matrix (ECM) through integrins and activation of small RhoGTPases, such as RhoA and Rac1, resulting in changes to actomyosin organization. During invasion, epithelial-derived tumor cells switch from lamininenriched basal membrane to collagen and fibronectin-enriched connective tissue. How this switch affects the tumor migration is still unclear. We tested the hypothesis that ECM dictates the invasiveness of Oral Squamous Cell Carcinoma (OSCC). We analyzed the migratory properties of two OSCC lines, a low invasive cell line with high e-cadherin levels (Linv/HE-cad) or a highly invasive cell line with low e-cadherin levels (Hinv/LE-cad), plated on different ECM components. Compared to laminin, fibronectin induced non-directional collective migration and decreased RhoA activity in Linv/HE-cad OSCC. For Hinv/LE-cad OSCC, fibronectin increased Rac1 activity and induced smaller adhesions, resulting in a fast single cell migration in both 2D and 3D environments. Consistent with these observations, human OSCC biopsies exhibited similar changes in cell-ECM adhesion distribution at the invasive front of the tumor, where cells encounter fibronectin. Our results indicate that ECM composition might induce a switch from collective to single cell migration according to tumor invasiveness due to changes in cell-ECM adhesion and the resulting signaling pathways that alter actomyosin organization.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofPLoS ONE. San Francisco. Vol. 11, no. 3 (2016), e0151338, 18 p.pt_BR
dc.rightsOpen Accessen
dc.subjectCarcinoma de células escamosaspt_BR
dc.subjectMatriz extracelularpt_BR
dc.subjectFibronectinaspt_BR
dc.titleFibronectin modulates cell adhesion and signaling to promote single cell migration of highly invasive oral squamous cell carcinomapt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001008772pt_BR
dc.type.originEstrangeiropt_BR


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