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dc.contributor.authorVisioli, Fernandapt_BR
dc.contributor.authorLauxen, Isabel da Silvapt_BR
dc.contributor.authorSant'Ana Filho, Manoelpt_BR
dc.contributor.authorRados, Pantelis Varvakipt_BR
dc.date.accessioned2012-11-21T01:51:12Zpt_BR
dc.date.issued2012pt_BR
dc.identifier.issn1678-7757pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/61210pt_BR
dc.description.abstractObjectives: The aim of this study was to analyze the immunolabeling of two cell cycle protein regulators, p53 and p21WAF1, in non-dysplastic leukoplakias with different epithelial alterations: acanthosis, hyperkeratosis and acanthosis combined with hyperkeratosis, and compare them with dysplastic leukoplakias. Material and Methods: This was a prospective cohort study involving 36 patients with oral homogeneous leukoplakias. Excisional biopsies were performed and the patients remain under clinical follow-up. The leukoplakias were divided into four groups: 6 acanthosis, 9 hyperkeratosis, 10 acanthosis combined with hyperkeratosis, and 11 epithelial dysplasias. Paraffin-embebeded sections were immunostained for p53 and p21WAF1. Five hundred cells from the basal layer and 500 from the parabasal layer were counted to determine the percentage of positive cells. A qualitative analysis was also carried out to determine the presence or absence of immunohistochemical staining in the intermediate and superficial layers. Groups were compared with ANOVA (p<0.05). Pearson’s correlation coefficient was used to test for associations between the two markers, p53 and p21WAF1. Results: No leukoplakia recurred and no malignant transformation was observed whitin a follow-up period of 3-6 years. The mean percentage of p53 staining in the basal and parabasal layers was similar in all groups. p21WAF1 staining differed between layers was as follows: in the basal, only 3 to 4% of cells were stained, while in the parabasal, between 16 and 28% of the epithelial cells were stained in the four different studied groups with no statistically significant difference (p>0.05). Conclusions: Our findings failed to differentiate the non-dysplastic lesions by means of p53 and p21WAF1 immunostaining, notwithstanding similar profiles between nondysplastic and dysplastic leukoplakias were observed.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofJournal of applied oral science. Bauru. Vol. 20, no. 3 (maio/jun. 2012), p. 369-375pt_BR
dc.rightsOpen Accessen
dc.subjectLeukoplakiaen
dc.subjectPatologia bucalpt_BR
dc.subjectCarcinoma bucalpt_BR
dc.subjectOral canceren
dc.subjectTumor suppressor protein p53en
dc.subjectCyclin-cependent Kinase inhibitor p21en
dc.titleExpression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakiaspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000860051pt_BR
dc.type.originNacionalpt_BR


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