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dc.contributor.authorCancela, Martínpt_BR
dc.contributor.authorRuétalo, Nataliapt_BR
dc.contributor.authorDell'Oca, Nicoláspt_BR
dc.contributor.authorSilva, Edileuza Danieli dapt_BR
dc.contributor.authorSmircich, Pablopt_BR
dc.contributor.authorRinaldi, Gabrielpt_BR
dc.contributor.authorRoche, Ledapt_BR
dc.contributor.authorCarmona, Carlos Albertopt_BR
dc.contributor.authorAlvarez Valín, Fernandopt_BR
dc.contributor.authorZaha, Arnaldopt_BR
dc.contributor.authorTort, Jose F.pt_BR
dc.date.accessioned2011-07-27T06:00:50Zpt_BR
dc.date.issued2010pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/30386pt_BR
dc.description.abstractBackground: The common liver fluke Fasciola hepatica is the agent of a zoonosis with significant economic consequences in livestock production worldwide, and increasing relevance to human health in developing countries. Although flukicidal drugs are available, re-infection and emerging resistance are demanding new efficient and inexpensive control strategies. Understanding the molecular mechanisms underlying the host-parasite interaction provide relevant clues in this search, while enlightening the physiological adaptations to parasitism. Genomics and transcriptomics are still in their infancy in F. hepatica, with very scarce information available from the invasive newly excysted juveniles (NEJ). Here we provide an initial glimpse to the transcriptomics of the NEJ, the first stage to interact with the mammalian host. Results: We catalogued more than 500 clusters generated from the analysis of F. hepatica juvenile expressed sequence tags (EST), several of them not detected in the adult stage. A set of putative F. hepatica specific transcripts, and a group of sequences conserved exclusively in flatworms were identified. These novel sequences along with a set of parasite transcripts absent in the host genomes are putative new targets for future anti-parasitic drugs or vaccine development. Comparisons of the F. hepatica sequences with other metazoans genomes or EST databases were consistent with the basal positioning of flatworms in the bilaterian phylogeny. Notably, GC content, codon usage and amino acid frequencies are remarkably different in Schistosomes to F. hepatica and other trematodes. Functional annotation of predicted proteins showed a general representation of diverse biological functions. Besides proteases and antioxidant enzymes expected to participate in the early interaction with the host, various proteins involved in gene expression, protein synthesis, cell signaling and mitochondrial enzymes were identified. Differential expression of secreted protease gene family members between juvenile and adult stages may respond to different needs during host colonization. Conclusion: The knowledge of the genes expressed by the invasive stage of Fasciola hepatica is a starting point to unravel key aspects of this parasite's biology. The integration of the emerging transcriptomics, and proteomics data and the advent of functional genomics tools in this organism are positioning F. hepatica as an interesting model for trematode biology.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBMC Genomics. London. Vol. 11, (Apr. 2010), p. 1-14pt_BR
dc.rightsOpen Accessen
dc.subjectFasciola hepaticapt_BR
dc.titleSurvey of transcripts expressed by the invasive juvenile stage of the liver fluke Fasciola hepaticapt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000762572pt_BR
dc.type.originEstrangeiropt_BR


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