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dc.contributor.authorZeb, Ismailpt_BR
dc.contributor.authorParizi, Luis Fernandopt_BR
dc.contributor.authorIsrar, Muhammadpt_BR
dc.contributor.authorVaz Junior, Itabajara da Silvapt_BR
dc.contributor.authorAli, Abidpt_BR
dc.date.accessioned2024-02-27T04:58:03Zpt_BR
dc.date.issued2024pt_BR
dc.identifier.issn1756-3305pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/272215pt_BR
dc.description.abstractBackground: Tick control is mostly hampered by the rise of acaricide-resistant tick populations. Significant efforts have focused on developing alternative control methods, including cross-species protective and/or cocktail-based anti-tick vaccines, to achieve protection against various tick species. Methods: In this study, full-length open reading frames encoding subolesin (SUB) from Rhipicephalus microplus and ferritin 2 (FER2) from Hyalomma anatolicum as well as the partial 60S acidic ribosomal protein (P0) from R. microplus were cloned, expressed in Escherichia coli and used as vaccine antigens against Rhipicephalus sanguineus sensu lato (R. sanguineus s.l.) infestation in rabbits. Results: In silico analyses revealed that the SUB, P0 and FER2 proteins were antigenic and displayed limited similarity to the host’s homologous proteins. The proteins shared identities of 97.5%, 100% and 89.5% with their SUB, P0 and FER2 R. sanguineus s.l. orthologous sequences, respectively. Antibodies against each recombinant protein cross-recognized the native proteins in the different tissues and developmental stages of R. sanguineus s.l. Overall efficacy of the SUB, FER2 and cocktail (SUB+FER2+P0) vaccines against R. sanguineus s.l. infestation was 86.3%, 95.9% and 90.9%, respectively. Conclusions Both mono-antigen and the cocktail anti-tick vaccines affected the biological parameters of R. sanguineus s.l. infestation in the rabbit model, which could be extrapolated to its infested host under natural conditions. These findings support the possibility of using mono-antigenic and cocktail-based vaccines for large-scale anti-tick vaccine development against multiple tick species.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofParasites & Vectors. London. Vol. 17 (2024), 3, 15 p.pt_BR
dc.rightsOpen Accessen
dc.subjectAnti-tick vaccineen
dc.subjectSubolesinapt_BR
dc.subjectCross-protectionen
dc.subjectFerritina 2pt_BR
dc.subjectProteínas ribossômicaspt_BR
dc.subjectCocktailen
dc.subjectAntigenospt_BR
dc.subjectRhipicephalus sanguineus sensu latoen
dc.subjectDesenvolvimento de vacinaspt_BR
dc.subjectProteção cruzadapt_BR
dc.subjectCarrapatospt_BR
dc.titleCross-species immunoprotective antigens (subolesin, ferritin 2 and P0) provide protection against Rhipicephalus sanguineus sensu latopt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001194266pt_BR
dc.type.originEstrangeiropt_BR


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