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dc.contributor.authorFerrarese, Patricia Aline Gröspt_BR
dc.contributor.authorStreit, Rodrigo Silva Araujopt_BR
dc.contributor.authorSantos, Patrícia Ribeiro dospt_BR
dc.contributor.authorSantos, Francine Melise dospt_BR
dc.contributor.authorAlmeida, Rita Maria Cunha dept_BR
dc.contributor.authorSchrank, Augustopt_BR
dc.contributor.authorSilva, Lívia Kmetzsch Rosa ept_BR
dc.contributor.authorVainstein, Marilene Henningpt_BR
dc.contributor.authorStaats, Charley Christianpt_BR
dc.date.accessioned2023-12-16T03:24:32Zpt_BR
dc.date.issued2017pt_BR
dc.identifier.issn2076-2607pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/268459pt_BR
dc.description.abstractCryptococcus gattii is a human and animal pathogen that infects healthy hosts and caused the Pacific Northwest outbreak of cryptococcosis. The inhalation of infectious propagules can lead to internalization of cryptococcal cells by alveolar macrophages, a niche in which C. gattii cells can survive and proliferate. Although the nutrient composition of macrophages is relatively unknown, the high induction of amino acid transporter genes inside the phagosome indicates a preference for amino acid uptake instead of synthesis. However, the presence of countable errors in the R265 genome annotation indicates significant inhibition of transcriptomic analysis in this hypervirulent strain. Thus, we analyzed RNA-Seq data from in vivo and in vitro cultures of C. gattii R265 to perform the reannotation of the genome. In addition, based on in vivo transcriptomic data, we identified highly expressed genes and pathways of amino acid metabolism that would enable C. gattii to survive and proliferate in vivo. Importantly, we identified high expression in three APC amino acid transporters as well as the GABA permease. The use of amino acids as carbon and nitrogen sources, releasing ammonium and generating carbohydrate metabolism intermediaries, also explains the high expression of components of several degradative pathways, since glucose starvation is an important host defense mechanism.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofMicroorganisms. Basel. Vol. 5, n. 3, (2017), 49, 15 p.pt_BR
dc.rightsOpen Accessen
dc.subjectCryptococcus gattiipt_BR
dc.subjectTranscriptomeen
dc.subjectAmino aciden
dc.subjectTranscriptomapt_BR
dc.subjectCriptococosept_BR
dc.subjectBronchoalveolar lavageen
dc.subjectTranscriptomeen
dc.subjectCryptococcosisen
dc.titleTranscriptional analysis allows genome reannotation and reveals that Cryptococcus gattii VGII undergoes nutrient restriction during infectionpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001072369pt_BR
dc.type.originEstrangeiropt_BR


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