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dc.contributor.authorAlves, Joana Silvapt_BR
dc.contributor.authorGarcia-Galan, Cristinapt_BR
dc.contributor.authorSchein, Mirela Ferreirapt_BR
dc.contributor.authorSilva, Alexandre Martins dapt_BR
dc.contributor.authorBarbosa, Oveimarpt_BR
dc.contributor.authorAyub, Marco Antônio Záchiapt_BR
dc.contributor.authorFernandez-Lafuente, Robertopt_BR
dc.contributor.authorRodrigues, Rafael Costapt_BR
dc.date.accessioned2023-12-14T03:23:55Zpt_BR
dc.date.issued2014pt_BR
dc.identifier.issn1420-3049pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/268389pt_BR
dc.description.abstractIt is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB). The present research evaluated the synthesis of butyl acetate using MCI-CALB under ultrasonic energy, comparing the results against those obtained using the commercial preparation, Novozym 435. The optimal conditions were determined using response surface methodology (RSM) evaluating the following parameters: reaction temperature, substrate molar ratio, amount of biocatalyst, and added water. The optimal conditions for butyl acetate synthesis catalyzed by MCI-CALB were: temperature, 48.8 °C; substrate molar ratio, 3.46:1 alcohol:acid; amount of biocatalyst, 7.5%; and added water 0.28%, both as substrate mass. Under these conditions, 90% of conversion was reached in 1.5 h. In terms of operational stability, MCI-CALB was reused in seven cycles while keeping 70% of its initial activity under ultrasonic energy. The support pore size and resistance are key points for the enzyme activity and stability under mechanical stirring. The use of ultrasound improved both activity and stability because of better homogeneity and reduced mechanical stress to the immobilized system.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofMolecules. Basel, Switzerland. Vol. 19, no.7 (July 2014), p. 9562-9576pt_BR
dc.rightsOpen Accessen
dc.subjectEsterificaçãopt_BR
dc.subjectEsterificationen
dc.subjectUtrasounden
dc.subjectLipasept_BR
dc.subjectUltrassompt_BR
dc.subjectEnzyme reuseen
dc.subjectEnzyme stabilityen
dc.subjectEnzimapt_BR
dc.subjectButyl acetateen
dc.titleCombined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by CALBpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000987544pt_BR
dc.type.originEstrangeiropt_BR


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