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dc.contributor.authorLoeuille, Benoitpt_BR
dc.contributor.authorThode, Verônica Aydospt_BR
dc.contributor.authorSiniscalchi, Carolina Morianipt_BR
dc.contributor.authorAndrade, Sónia Cristina da Silvapt_BR
dc.contributor.authorRossi, Maria Magdalenapt_BR
dc.contributor.authorPirani, Jose Rubenspt_BR
dc.date.accessioned2023-11-30T03:23:11Zpt_BR
dc.date.issued2021pt_BR
dc.identifier.issn2167-8359pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/267820pt_BR
dc.description.abstractAldama (Heliantheae, Asteraceae) is a diverse genus in the sunflower family. To date, nearly 200 Asteraceae chloroplast genomes have been sequenced, but the plastomes of Aldama remain undescribed. Plastomes in Asteraceae usually show little sequence divergence, consequently, our hypothesis is that species of Aldama will be overall conserved. In this study, we newly sequenced 36 plastomes of Aldama and of five species belonging to other Heliantheae genera selected as outgroups (i.e., Dimerostemma asperatum, Helianthus tuberosus, Iostephane heterophylla, Pappobolus lanatus var. lanatus, and Tithonia diversifolia). We analyzed the structure and gene content of the assembled plastomes and performed comparative analyses within Aldama and with other closely related genera. As expected, Aldama plastomes are very conserved, with the overall gene content and orientation being similar in all studied species. The length of the plastome is also consistent and the junction between regions usually contain the same genes and have similar lengths. A large ~20 kb and a small ~3 kb inversion were detected in the Large Single Copy (LSC) regions of all assembled plastomes, similarly to other Asteraceae species. The nucleotide diversity is very low, with only 1,509 variable sites in 127,466 bp (i.e., 1.18% of the sites in the alignment of 36 Aldama plastomes, with one of the IRs removed, is variable). Only one gene, rbcL, shows signatures of positive selection. The plastomes of the selected outgroups feature a similar gene content and structure compared to Aldama and also present the two inversions in the LSC region. Deletions of different lengths were observed in the gene ycf2. Multiple SSRs were identified for the sequenced Aldama and outgroups. The phylogenetic analysis shows that Aldama is not monophyletic due to the position of the Mexican species A. dentata. All Brazilian species form a strongly supported clade. Our results bring new understandings into the evolution and diversity of plastomes at the species level.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofPeerJ. Corte Madera. Vol. 9 (2021), e10886, 36 p.pt_BR
dc.rightsOpen Accessen
dc.subjectComplete plastomeen
dc.subjectCompositaept_BR
dc.subjectYcf2 geneen
dc.subjectRbcL positive selectionen
dc.subjectPlastome evolutionen
dc.titleExtremely low nucleotide diversity among thirty-six new chloroplast genome sequences from Aldama (Heliantheae, Asteraceae) and comparative chloroplast genomics analyses with closely related generapt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001176357pt_BR
dc.type.originEstrangeiropt_BR


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